Influence of Stroke on the Composition of Intestinal Microbiota (GUTSTROKE)
The aim of the study is to verify the hypothesis that the microbial colonisation of the gut is changed in patients after stroke and that the gut microbiome of severely affected stroke patients differs from that of patients with only a short disruption of blood circulation in the brain (transient ischemic attack, TIA). For this, the composition of gut microbiota in stool samples will be analyzed by 454 pyrosequencing. Further, the correlation of stroke-associated changes in the microbiome with immunological parameters will be analyzed.
Transient Ischemic Attack (TIA)
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Influence of Stroke on the Composition of Intestinal Microbiota (GUTSTROKE)|
- composition of intestinal microbiome [ Time Frame: change between day 1, day 4, day 7, day 14 after stroke ] [ Designated as safety issue: No ]The composition of the intestinal metagenome / microbiome will be determined by analyzing the 16S rRNA by 454 Pyrosequencing.
- test of correlation between most changed group of bacteria with changes in immunological parameters (HLA-DR) [ Time Frame: days 1, 4, 7 after stroke ] [ Designated as safety issue: No ]Analysis of correlation between changes in microbiome (most changed group of bacteria, e.g. lactobacilli) with changes in immunological parameters (e.g. HLA-DR, IL-6, IL-10, CD4-lymphocytes from blood samples).
Biospecimen Retention: Samples With DNA
Stool samples, Blood samples
|Study Start Date:||May 2014|
|Estimated Study Completion Date:||December 2015|
|Estimated Primary Completion Date:||December 2015 (Final data collection date for primary outcome measure)|
patients with a transient ischemic attack (control group)
Please refer to this study by its ClinicalTrials.gov identifier: NCT02008604
|Contact: Andreas Meisel, Prof. Dr.||email@example.com|
|Contact: Katarzyna Winekfirstname.lastname@example.org|
|Charite Universitätsmedizin Berlin||Recruiting|
|Berlin, Germany, 10117|
|Contact: Andreas Meisel, Prof. Dr. 0049 30 450 660026 email@example.com|
|Contact: Katarzyna Winek firstname.lastname@example.org|
|Principal Investigator:||Andreas Meisel, Prof. Dr.||Charite University, Berlin, Germany|