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Exercise Effects on Insulin, Gut Peptides, and Appetite (XFG)

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ClinicalTrials.gov Identifier: NCT01891617
Recruitment Status : Completed
First Posted : July 3, 2013
Last Update Posted : June 13, 2016
Sponsor:
Collaborator:
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Information provided by (Responsible Party):
Dr. Katarina Borer, University of Michigan

Brief Summary:
Determine whether the mid-day suppression of hunger and amplified increase in the release of glucose-dependent insulinotropic peptide (GIP) and glucagon-like peptide 1 (GLP-1) following morning exercise is due to increased fat content of the diet per se or a combination of high fat diet after morning exercise. The action of gut peptides, particularly GLP-1, on gastric emptying is likely to be important in mediating its effects on postprandial appetite and glycemia (Nauck et al. 1997). Our hypothesis is that exercise amplifies gut peptide secretion when diet is enriched with fat, and that this stimulus suppresses the hunger sensation.

Condition or disease Intervention/treatment Phase
Endocrine and Metabolic Responses to Exercise and Diets Other: Exercise and diets Not Applicable

Detailed Description:

Specific aim: Determine whether a change in macronutrient composition from 60% carbohydrate and 25% fat to 30% carbohydrate and 45% fat is responsible by itself for suppression of hunger and increased secretory response of glucose-dependent insulinotropic hormone (GIP) and glucagon-like peptide-1 (GLP-1), or whether these changes depend on preceding exercise. We will measure (a) concentrations of plasma GIP and GLP-1 by chemiluminescent multiplex assay, (b) concentrations of plasma ancetaminophen to assess the gastric emptying rate, (c) concentrations of plasma insulin, and glucagon by radioimmunoassay, and glucose, ketone bodies, and free fatty acids with appropriate spectrophotometric methods, (d) hourly appetite responses with visual analog scale under two conditions: sedentary (SED) and exercise (EX).

Hypothesis: Hunger suppression and secretion of GIP and GLP-1 after the morning meal will be greater with slower gastric emptying rate when a meal consisting of 45% fat and 30% carbohydrate follows three hours after a 2-hour bout of moderate-intensity exercise than in the absence of exercise.


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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 16 participants
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: The Exercise Effects on Appetite and Gut Peptides While on High-fat Diet
Study Start Date : November 2010
Actual Primary Completion Date : July 2012
Actual Study Completion Date : March 2013

Resource links provided by the National Library of Medicine


Arm Intervention/treatment
Experimental: Exercise-high-fat diet
Two bouts of exercise followed by a high-fat meal
Other: Exercise and diets
Exercise and high-fat diet Exercise and high-carbohydrate diet Sedentary and high-fat diet Sedentary and high-carbohydrate diet

Experimental: Exercise-high-carbohydrate diet
Two bouts of exercise followed by a high-carbohydrate meal
Other: Exercise and diets
Exercise and high-fat diet Exercise and high-carbohydrate diet Sedentary and high-fat diet Sedentary and high-carbohydrate diet

Experimental: Sedentary-high-fat diet
Sedentary trial with two high-fat meals
Other: Exercise and diets
Exercise and high-fat diet Exercise and high-carbohydrate diet Sedentary and high-fat diet Sedentary and high-carbohydrate diet

Experimental: Sedentary-high-carbohydrate diet
Sedentary trial with two high-carbohydrate meals
Other: Exercise and diets
Exercise and high-fat diet Exercise and high-carbohydrate diet Sedentary and high-fat diet Sedentary and high-carbohydrate diet




Primary Outcome Measures :
  1. Plasma insulin concentration [ Time Frame: 36 hours ]
    Change in plasma insulin to two bouts of exercise followed by a high-carbohydrate or a high fat diet

  2. Plasma glucagon concentration [ Time Frame: 36 hours ]
    Changes in plasma glucagon concentration to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal

  3. Plasma concentration of glucose-dependent insulinotropic peptide (GIP) [ Time Frame: 36 hours ]
    Changes in plasma concentration of glucose-dependent insulinotropic peptide (GIP)to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal

  4. Plasma concentration of free fatty acids [ Time Frame: 36 hours ]
    Changes in plasma concentration of free fatty acidsto two bouts of exercise followed by either a high-carbohydrate or a high-fat meal

  5. Plasma concentration of beta-hydroxybutyrate [ Time Frame: 36 hours ]
    Changes in plasma concentration of beta-hydroxybutyrate to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal

  6. Plasma glucose concentration [ Time Frame: 36 hours ]
    Changes in plasma glucose concentration to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal

  7. daytime hourly appetite ratings [ Time Frame: 36 hours ]
    Changes in daytime hourly appetite ratings to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal


Secondary Outcome Measures :
  1. Plasma concentrations of glucagon-like peptide-1 (GLP-1) [ Time Frame: 36 hours ]
    Changes in plasma concentrations of glucagon-like peptide-1 (GLP-1)to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal

  2. Plasma concentrations of peptide tyrosine tyrosine (PYY) [ Time Frame: 36 hours ]
    Changes in plasma concentrations of peptide tyrosine tyrosine (PYY) to two bouts of exercise followed by either a high-carbohydrate or a high-fat meal



Information from the National Library of Medicine

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Ages Eligible for Study:   50 Years to 65 Years   (Adult, Older Adult)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Postmenopausal status
  • Age 50 to 65 years
  • BMI between 20 and 30 kg/m2.
  • Good health status (normotensive, fasting glucose < 100 mg/dl, , hematocrit > 32%, hemoglobin >12 mg/dl)
  • Absence of restricted food intake
  • Absence of endocrine and metabolic disorders requiring medication other than hormonally corrected hypothyroidism
  • Absence of musculoskeletal disabilities that would prevent walking

Exclusion Criteria:

  • Presence of endocrine and metabolic disease requiring medication, other than hormonally corrected hypothyroidism
  • Presence of musculoskeletal disabilities that would prevent walking
  • Smoking
  • Active dieting
  • Absence of listed inclusion criteria
  • Unwillingness to follow study protocol.

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01891617


Locations
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United States, Michigan
Michigan Clinical Research Unit
Ann Arbor, Michigan, United States, 48109
Sponsors and Collaborators
University of Michigan
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Investigators
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Principal Investigator: Katarina Borer, PhD University of Michigan

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Responsible Party: Dr. Katarina Borer, Professor, University of Michigan
ClinicalTrials.gov Identifier: NCT01891617     History of Changes
Other Study ID Numbers: R15DK082800-XFG
R15DK082800 ( U.S. NIH Grant/Contract )
First Posted: July 3, 2013    Key Record Dates
Last Update Posted: June 13, 2016
Last Verified: June 2016

Keywords provided by Dr. Katarina Borer, University of Michigan:
exercise
high carbohydrate and high fat diets
insulin
gut peptides
appetite
metabolism
glucose