Epigenetics and the Origin of Muscle Insulin Resistance in Humans - Full Text View

Purpose

The investigators are trying to understand the role of DNA (deoxyribonucleic acid) methylation in insulin resistance in skeletal muscle and blood tissues. DNA methylation is a normal chemical process in the body that modifies DNA. By studying this, the investigators hope to better understand the causes of insulin resistance.

Condition
Diabetes Mellitus Type 2 in Obese Obesity


Study Type:	Observational
Study Design:	Observational Model: Cohort Time Perspective: Prospective
Official Title:	Epigenetics and the Origin of Muscle Insulin Resistance in Humans

Resource links provided by NLM:

Drug Information available for: Insulin Insulin human

U.S. FDA Resources

Further study details as provided by Mayo Clinic:

Primary Outcome Measures:

DNA methylation of genes in insulin resistance [ Time Frame: Baseline to visit 33 (approx 2 months) ]
DNA methylation of genes involved in mitochondrial biogenesis, oxidative phosphorylation, extracellular matrix and cytoskeleton proteins in insulin resistance, with an acute episode of exercise, and with eight weeks of training exercise.

Secondary Outcome Measures:

mRNA expression of genes [ Time Frame: Baseline to visit 33 approx 2 months ]
mRNA expression of genes involved in mitochondrial biogenesis, oxidative phosphorylation, extracellular matrix and cytoskeletal signaling are altered in insulin resistance, with an acute episode of exercise and with 8 weeks of exercise training.

Biospecimen Retention: Samples With DNA

thigh muscle biopsies bloodsamples


Estimated Enrollment:	72
Study Start Date:	August 2012
Estimated Study Completion Date:	August 2017
Estimated Primary Completion Date:	August 2017 (Final data collection date for primary outcome measure)

Groups/Cohorts
Insulin resistance epigenetics This experiment will use the Infinium methylation assay to perform epigenome mapping and define patterns of DNA methylation in skeletal muscle and whole blood tissue of metabolically well-characterized lean healthy, obese nondiabetic, and type 2 diabetic volunteers. We will test the hypotheses that (1) There is an increased methylation of genes involved in mitochondrial biogenesis and oxidative phosphorylation and altered methylation of promoters of genes coding for extracellular matrix and cytoskeletal proteins in insulin resistance, (2) The altered methylation patterns observed correspond to protein and mRNA expression changes, and (3) There are coordinated patterns of DNA methylation between the skeletal muscle and whole blood tissues in insulin resistance.
Single bout of exercise This experiment will test the hypotheses in lean healthy, obese non-diabetic and type 2 diabetic volunteers that Increased methylation of the PGC-1α promoter predicts a decreased response of this gene to a single bout of exercise, and Altered methylation of promoters of nuclear encoded mitochondrial genes predicts a decreased response of this gene to a single bout of exercise.
Eight weeks of exercise This experiment will test the hypothesis in lean healthy, obese non-diabetic and type 2 diabetic volunteers that There is decreased methylation of genes involved in mitochondrial biogenesis and oxidative phosphorylation, and the altered methylation corresponds to protein and mRNA (messenger ribonucleic acid) expression changes, There is altered methylation of genes involved in inflammation and cytoskeletal structure.

Detailed Description:

Insulin resistance is defined as the decreased ability of insulin to perform its biological function in the muscle, liver and fat. Genetic and environmental factors are known to influence insulin sensitivity. It is not known how this is mediated. This study looks at the role of epigenetics (modifications of proteins associated with DNA and methylation of DNA) in alterations in insulin resistance. We will study lean healthy people, obese non-diabetic people and people with type 2 diabetes to characterize the DNA methylation patterns in muscle in each group. The second aim of the study is to see how a single bout of exercise affects the DNA methylation in the muscle. The third aim looks at the effect of 8 weeks of supervised exercise on the DNA methylation.

Eligibility


Ages Eligible for Study:	21 Years to 55 Years (Adult)
Sexes Eligible for Study:	All
Accepts Healthy Volunteers:	Yes
Sampling Method:	Non-Probability Sample

Study Population

Three groups of volunteers will be studied: 1) lean, healthy volunteers, 2)obese volunteers without type 2 diabetes, and 3) volunteers with type 2 diabetes.

Criteria

Volunteers must be:

21 - 55 years old
must be non-lactating, non-pregnant
not taking medications known to affect glucose or if taking them, on stable doses.
free of significant heart or lung disease

Contacts and Locations

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01726491

Contacts


Contact: Charlene Robinson	480-301-4626	ARZClinicalStudies@mayo.edu

Locations


United States, Arizona
Mayo Clinic in Arizona	Recruiting
Scottsdale, Arizona, United States, 85259
Contact: Charlene Robinson 480-301-4626 ARZClinicalStudies@mayo.edu

Sponsors and Collaborators

Mayo Clinic

Investigators


Principal Investigator:	Lori Roust, MD	Mayo Clinic
Principal Investigator:	Dawn K Coletta, Ph.D.	Mayo Clinic

More Information


Responsible Party:	Lori R. Roust, Consultant in Endocrinology, Mayo Clinic
ClinicalTrials.gov Identifier:	NCT01726491 History of Changes
Other Study ID Numbers:	11-007028
Study First Received:	November 8, 2012
Last Updated:	October 27, 2016

Keywords provided by Mayo Clinic:


Insulin resistance Exercise Type 2 diabetes mellitus Obesity Epigenetic studies

Additional relevant MeSH terms:


Diabetes Mellitus Insulin Resistance Diabetes Mellitus, Type 2 Glucose Metabolism Disorders Metabolic Diseases Endocrine System Diseases	Hyperinsulinism Insulin, Globin Zinc Insulin Hypoglycemic Agents Physiological Effects of Drugs

ClinicalTrials.gov processed this record on May 25, 2017