IL8 Monitoring and Its Correlation With 251-gene Polymorphism
|ClinicalTrials.gov Identifier: NCT01708759|
Recruitment Status : Completed
First Posted : October 17, 2012
Last Update Posted : April 8, 2014
|Condition or disease|
|Sepsis Systemic Inflammatory Response Syndrome|
|Study Type :||Observational|
|Actual Enrollment :||80 participants|
|Observational Model:||Case Control|
|Official Title:||The Value of Admission Serum Il-8 Monitoring and the Detection of IL8 (-251 A/T) Polymorphism in Critically Ill Patients|
|Study Start Date :||November 2012|
|Actual Primary Completion Date :||February 2013|
|Actual Study Completion Date :||February 2013|
Forty patients developed septic complication during ICU stay (sepsis group).
Forty patients were critically ill without evidence of infectious organism (SIRS group).
- Serum Interlukin-8 [ Time Frame: At intensive care admission. Patients will be followed for at least 2 weeks ]Serum level of IL-8 was determined by quantitative sandwich enzyme immunoassay (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer's instructions. The intensity of the colour was measured at 490 nm for IL-8.
- Interlukine-8polymorphism at 251 A/t [ Time Frame: At ICU admission. Patients will be followed for at least 2weeks. ]About 4ml blood is withdrawn in EDTA-tubes and the genomic DNA was extracted. Concentration of the extracted DNA was then measured by UV spectrophotometry at 260& 280 nm and run on agaros gel electrophoresis 2% for detection of purity. Molecular detection of the (-251 A/T) polymorphism in the IL-8gene was achieved by restriction fragment length polymorphism typing.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01708759
|Faculty of Medicine ,Tanta University.|
|Tanta, Algharbiya, Egypt, 35217|
|Principal Investigator:||Ayman A Yousef, MD||Assistant professor os Anesthesia and ICU, Faculty of Medicine, Tanta university|