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The Effects of Amino Acid Supplement During Acute Inflammation.

This study has been completed.
Information provided by (Responsible Party):
Nikolaj Fibiger Rittig, University of Aarhus Identifier:
First received: October 8, 2012
Last updated: November 11, 2014
Last verified: November 2014

The purpose of the study is to investigate the metabolic effects during acute inflammation with and without the nutritional supplement of amino acids.

E. Coli Endotoxin (LPS, lipopolysaccharide) is used to initiate an inflammatory response.

The study is an interventional randomized placebo study including 8 healthy male subjects. Each subject participates 3 times (different days) and are given one of following interventions:

  • Placebo (NaCl)
  • Endotoxin, US standard reference E.Coli + Placebo (NaCl)
  • Endotoxin, US standard reference E.Coli + Amino acids (intravenously)

It is our hypothesis that the nutritional intervention during acute inflammation plays an important role in lipid and protein metabolism.

Condition Intervention
Febrile Illness Acute Inflammation Metabolic Diseases Biological: E. coli endotoxin, US standard

Study Type: Interventional
Study Design: Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single (Participant)
Primary Purpose: Treatment
Official Title: Metabolic Effects of Endotoxin Induced Acute Inflammation in Healthy Young Men With and Without Supplement of Amino Acids.

Resource links provided by NLM:

Further study details as provided by Nikolaj Fibiger Rittig, University of Aarhus:

Primary Outcome Measures:
  • Protein catabolism during acute illness [ Time Frame: After 6 hours intervention ]
    Protein catabolism is measured with amino acid tracer techniques comparing radioactive marked amino acids from blood samples.

Secondary Outcome Measures:
  • The local effects on muscle and fat tissue [ Time Frame: At 2 hours and 4,5 hours ]
    A muscle + fat biopsy are taken twice during the intervention to focus on the local effects in these tissues.

  • Other systemic metabolic effects during acute illness [ Time Frame: 6 hours ]
    Lipid and glucose metabolism are measured with tracer techniques with radioactive marked glucose + lipid.

Enrollment: 8
Study Start Date: October 2012
Study Completion Date: December 2013
Primary Completion Date: December 2013 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
No Intervention: Saline
Only saline is given.
Placebo Comparator: + Saline
Endotoxin is given + Placebo (Saline)
Biological: E. coli endotoxin, US standard
Active Comparator: + amino acids
Endotoxin is given + amino acids (intravenously)
Biological: E. coli endotoxin, US standard


Ages Eligible for Study:   25 Years and older   (Adult, Senior)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • male gender
  • 20<BMI<30
  • Age > 25 years old
  • Written consent before starting the study

Exclusion Criteria:

  • Allergy for soya products ore egg
  • Diabetes
  • Epilepsy
  • Infection
  • Immune defects
  • Heart disease
  • Dysregulated hypertension
  • Participating in other trials using radioactive tracers or x-rays the last year.
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Please refer to this study by its identifier: NCT01705782

Medical Department MEA, NBG, Aarhus University Hospital
Aarhus C, Jylland, Denmark, 8000
Sponsors and Collaborators
University of Aarhus
  More Information

Publications automatically indexed to this study by Identifier (NCT Number):
Responsible Party: Nikolaj Fibiger Rittig, MD (medical graduate) and Ph.d.-student, University of Aarhus Identifier: NCT01705782     History of Changes
Other Study ID Numbers: 2012-410-12
Study First Received: October 8, 2012
Last Updated: November 11, 2014

Additional relevant MeSH terms:
Metabolic Diseases
Pathologic Processes processed this record on September 21, 2017