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Regulation of Tissue Lipolysis by Insulin in Type 2 Diabetes

This study has been completed.
Netherlands Organisation for Scientific Research
European Foundation for the Study of Diabetes
Information provided by (Responsible Party):
Maastricht University Medical Center Identifier:
First received: August 21, 2012
Last updated: October 11, 2012
Last verified: October 2012
Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.

Condition Intervention
Type 2 Diabetes
Other: Hyperinsulinemic euglycemic clamp

Study Type: Observational
Study Design: Observational Model: Case Control
Time Perspective: Cross-Sectional
Official Title: Regulation of Lipolysis by Insulin in Skeletal Muscle and Adipose Tissue in Type 2 Diabetes

Further study details as provided by Maastricht University Medical Center:

Primary Outcome Measures:
  • Change from baseline in insulin sensitivity of adipose tissue and skeletal muscle lipolysis [ Time Frame: 8, 20 and 40 mU insulin (2 hours) ]
    Changes from baseline in glycerol (lactate, pyruvate and glucose) in tissue microdialysate during a 3 step euglycemic hyperinsulinemic clamp (8, 20 and 40mU of insulin), every step for 2h

Secondary Outcome Measures:
  • Baseline adipose tissue and skeletal muscle gene expression [ Time Frame: baseline ]
    gene expression of genes related to lipid, glucose metabolism, insulin signalling will be measured in baseline adipose tissue and skeletal muscle biopsies

  • Baseline skeletal muscle lipid accumulation [ Time Frame: Baseline ]
    Baseline skeletal muscle lipid accumulation is measured (TAG, DAG, Phospholipid and fatty acids)

Biospecimen Retention:   Samples With DNA
adipose tissue and skeletal muscle biopsies

Enrollment: 20
Study Start Date: June 2007
Study Completion Date: September 2011
Primary Completion Date: September 2011 (Final data collection date for primary outcome measure)
Groups/Cohorts Assigned Interventions
Normal glycaemic healthy control men, age between 45-65, BMI 25-35 kg/m2
Other: Hyperinsulinemic euglycemic clamp
Type 2 diabetic men, age 45-65 yrs, BMI 25-35, diagnosed >5yrs and Hba1c 7-9%
Other: Hyperinsulinemic euglycemic clamp


Ages Eligible for Study:   45 Years to 65 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Sampling Method:   Probability Sample
Study Population
Healthy normoglycemic men and type 2 diabetic men

Inclusion Criteria:

  • Caucasian
  • Normal blood pressure (SBP 100-140 mmHg, DBP 60-90 mmHg)
  • weight stable in last 3 months

Exclusion Criteria:

  • Smokers
  • people with intensive fitness training (e.g. athletes > 3 times/week)
  • History of cardiovascular diseases
  • Bleeding disorders
  • Use of medication interfering with the study endpoints/hypotheses (e.g. beta-blockers)
  • Not to be able to understand the study information
  • Subjects on a special diet or vegetarian
  • Blood donation 2 months prior to the study and during the study
  • Participating in an other study
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Please refer to this study by its identifier: NCT01680133

Department of Human Biology, Maastricht University Medical Centre
Maastricht, Netherlands, 6200MD
Sponsors and Collaborators
Maastricht University Medical Center
Netherlands Organisation for Scientific Research
European Foundation for the Study of Diabetes
Principal Investigator: Ellen Blaak, PhD Maastricht University Medical centre
  More Information

Publications automatically indexed to this study by Identifier (NCT Number):
Responsible Party: Maastricht University Medical Center Identifier: NCT01680133     History of Changes
Other Study ID Numbers: 07-3-037
91611074 ( Other Grant/Funding Number: The Netherlands Organisation for Scientific Research )
Study First Received: August 21, 2012
Last Updated: October 11, 2012

Additional relevant MeSH terms:
Diabetes Mellitus
Diabetes Mellitus, Type 2
Glucose Metabolism Disorders
Metabolic Diseases
Endocrine System Diseases processed this record on April 28, 2017