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To Study Gene Expression of Postburn Hypertrophic Scar

The recruitment status of this study is unknown. The completion date has passed and the status has not been verified in more than two years.
Verified August 2012 by National Taiwan University Hospital.
Recruitment status was:  Recruiting
Information provided by (Responsible Party):
National Taiwan University Hospital Identifier:
First received: August 28, 2012
Last updated: NA
Last verified: August 2012
History: No changes posted
Aim: To compare the gene expression of postburn hypertrophic scar with normal skin.

Cicatrix, Hypertrophic

Study Type: Observational
Study Design: Observational Model: Case Control
Time Perspective: Prospective
Official Title: To Study Gene Expression of Postburn Hypertrophic Scar

Resource links provided by NLM:

Further study details as provided by National Taiwan University Hospital:

Biospecimen Retention:   Samples With DNA
Hypertrophic scar from post-burn patient

Estimated Enrollment: 30
Study Start Date: July 2012
Estimated Primary Completion Date: July 2013 (Final data collection date for primary outcome measure)
hypertrophic scar
1x1cm2 hypertrophic scar of post-burn patients are taked by visiting staff when they accept scar-reconstructive surgery.
normal skin
When the patient accept skin grafting surgery, visiting staff will take 1x1cm2 normal skin.

Detailed Description:

Background: Postburn hypertrophic scar (HS) remains a challenging problem. Although varied clinical therapeutic methods have been used, none of them has been widely accepted as a standard care. As hypertrophic scar is hereditary, gene therapy can be a potential tool for scar treatment. To investigate the correlation between the clinical presentation of the hypertrophic scar and its gene expression, we propose an analysis of gene expression in the hypertrophic scar using DNA microarray and Real-Time Quantitative PCR. The study result may form the base of clinical treatment.

Methods and Materials: Thirty scar patients requiring surgical treatment in NTUH burn center will be enrolled into the study. Inclusion criteria: (1) Age between 20 and 80 (2) Patients with postburn hypertrophic scar require surgical treatment.

  1. Hypertrophic scar and normal skin collection: Under Clinical Trial Informed Consent of Research Ethic Committee, scar and normal skin specimen of 1x1cm2 each will be collected and transported to tissue bank with a vacuum low temperature container. The specimen will be kept in a -80℃ refrigerator.
  2. RNA extraction from skin tissue: The RNA extraction will be performed using kit available in the market. Spectrophotometer and gel electrophoresis will be used to measure the purity and concentration of RNA.
  3. Gene expression analysis: DNA microarray and Real-Time Quantitative PCR will be used to analyze RNA expression of hypertrophic scar and normal skin.

Statistics: Student's t-test will be used to compare statistically gene expression between hypertrophic scar and normal skin. P < 0.05 will be considered as statistical significance.


Ages Eligible for Study:   20 Years to 80 Years   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
The patient have hypertrophic scar.

Inclusion Criteria:

  • age20~80, hypertrophic scar patient

Exclusion Criteria:

  • no specific limitation
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its identifier: NCT01676168

Contact: Kean Eng Yeong, college 886-2-23123456 ext 65648

National Taiwan University Hospital Recruiting
Taipei, Taiwan, 10002
Contact: Kean Eng Yeong, college    886-2-23123456 ext 65648   
Principal Investigator: Kean Eng Yeong, College         
Sponsors and Collaborators
National Taiwan University Hospital
Study Chair: Kean Eng Yeong, college NTUH Burn lab
  More Information

Responsible Party: National Taiwan University Hospital Identifier: NCT01676168     History of Changes
Other Study ID Numbers: 201205098RIC
Study First Received: August 28, 2012
Last Updated: August 28, 2012

Additional relevant MeSH terms:
Cicatrix, Hypertrophic
Pathological Conditions, Anatomical
Pathologic Processes processed this record on September 21, 2017