Nutrient Regulation of Amino Acid Transporters in Aging Human Skeletal Muscle
The goal of the research project is to determine how aging and inactivity reduce the muscle anabolic effect of nutrients and lead to muscle and functional loss. The central hypothesis is that aging reduces mTORC1 signaling and the expression of skeletal muscle amino acid transporters in response to anabolic stimulation leading to reduced muscle adaptation to increased intracellular amino acid requirements. The investigators further hypothesize that inactivity exacerbates this effect with significant muscle and functional loss, and rehabilitation restores muscle signaling, metabolism and function to baseline values.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Nutrient Regulation of Amino Acid Transporters in Aging Human Skeletal Muscle|
- amino acid transporter [ Time Frame: before and after (1 and 3h) amino acid ingestion. These samples will be taken before bed rest and 7 days after bed rest. ] [ Designated as safety issue: No ]Muscle biopsies will be sampled from the vastus laterals before amino acid ingestion and then 1 and 3h after amino acid ingestion. Muscle biopsies will be processed using western blotting and gene expression to assess the expression of specific amino acid transporters. The investigators are interested in the change in expression of amino acid transporters in response to amino acid ingestion from baseline. The response before bed rest will be compared to after bed rest.
|Study Start Date:||December 2011|
|Estimated Study Completion Date:||January 2017|
|Estimated Primary Completion Date:||September 2015 (Final data collection date for primary outcome measure)|
Please refer to this study by its ClinicalTrials.gov identifier: NCT01669590
|United States, Utah|
|The University of Utah|
|Salt Lake City, Utah, United States, 84112|