Signaling Pathway Activation After Exercise in Patients With Chronic Obstructive Pulmonary Disease (SIM)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT01561625
Recruitment Status : Completed
First Posted : March 23, 2012
Last Update Posted : May 7, 2013
Canadian Institutes of Health Research (CIHR)
Information provided by (Responsible Party):
Bruno Lemire, Laval University

Brief Summary:

Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD). Although resistance exercises increase strength and muscle mass in patients with COPD, the response to training appears to be suboptimal in these individuals. A dysregulation in the signaling pathways involved in the regulation of muscle mass could play an important role in this phenomenon.

Hypothesis: Proteins involved in muscle mass regulation will be less activated in the quadriceps of patients with COPD following the acute bout of resistance training exercise compared to healthy age-matched controls.

Condition or disease Intervention/treatment Phase
Chronic Obstructive Pulmonary Disease Other: exercise Not Applicable

Detailed Description:
Our objective is to investigate the impact of an acute bout of resistance training on key signaling pathways involved in the regulation of muscle mass in moderate to severe COPD (FEV1 under 60% of predicted). Key proteins of signaling intramuscular pathways involved in protein synthesis and degradation will be measured before after 72 contractions of the quadriceps. The exercise protocol was designed to match the actual exercise prescription model use in the context of pulmonary rehabilitation. A squat, leg press and leg extension will be done at 80% of max with one minute rest between sets. A biopsy of the quadriceps will be done before and after exercise in order to measure phosphorylated kinases, total protein content and mRNA expressions. All our patients will undergo a thorough baseline assessment in pulmonary capacity, muscle force and body composition.

Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 21 participants
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: Signaling Pathway Activation in the Quadriceps of Patients With COPD After an Acute Bout of Resistance
Study Start Date : October 2009
Actual Primary Completion Date : April 2013
Actual Study Completion Date : April 2013

Resource links provided by the National Library of Medicine

Intervention Details:
  • Other: exercise
    resistance training exercise, 1 session, 3 exercises, 80% of max

Primary Outcome Measures :
  1. Post-exercise signaling proteins phosphorylation level in the quadriceps of COPD patients and age-matched healthy controls [ Time Frame: 2 hours post-exercise ]
    Phosphorylation status of key proteins (Akt, p70, mTOR, p38, JNK, ERK) will be lesser modulated in the quadriceps of patients with COPD compare to healthy controls. The phosphorylated as well as the total protein levels will be measured by western blot. The data will be presented as arbitrary units and compared with values obtained in healthy age-matched healthy controls.

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Ages Eligible for Study:   50 Years to 75 Years   (Adult, Older Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Male
  • COPD with an FEV1 of under 60% of predicted
  • non-smoker
  • Between 50 and 75 years old

Exclusion Criteria:

  • All inflammatory disease (HIV, Cancer, renal and cardiac deficiency)
  • Hormonal dysregulation
  • Inferior limb pathology
  • Neuromuscular pathology
  • History of tabacco or alcool abuse
  • Oxygen dependent
  • Recent exacerbation (2 months) of the symptoms of COPD

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT01561625

Canada, Quebec
Quebec City, Quebec, Canada, G1V 4G5
Sponsors and Collaborators
Laval University
Canadian Institutes of Health Research (CIHR)
Principal Investigator: François Maltais, MD Laval University

Responsible Party: Bruno Lemire, Étudiant au doctorat, Laval University Identifier: NCT01561625     History of Changes
Other Study ID Numbers: SIM-COPD-20458
First Posted: March 23, 2012    Key Record Dates
Last Update Posted: May 7, 2013
Last Verified: May 2013

Keywords provided by Bruno Lemire, Laval University:
exercise training

Additional relevant MeSH terms:
Lung Diseases
Lung Diseases, Obstructive
Pulmonary Disease, Chronic Obstructive
Respiratory Tract Diseases