Compared Effect of a Fruit Milk Shake With a Protein-Carbohydrate Supplement on Recovery After Resistance Exercise
The aim of this study is to assess the effect of ingesting a commercial carbohydrate and protein dietary supplement in powder form (P-CHO supplement) or a milk shake with skimmed milk, strawberries and banana (MS), after resistance exercise, in muscle damage, oxidative stress, inflammation and functional recovery. It is hypothesised if the ingestion of a milk shake with skimmed milk and fruit (strawberry and banana) has the same impact on markers of muscle damage, oxidative stress, inflammation and functional recovery induced by resistance exercise, as the intake of a commercial powder with the same CHO and protein amounts.
Fifteen adult athletes from the Portuguese Athletic Federation will complete 2 trials separated at least by 2 weeks. Alternate legs and drinks will be used in each trial and participants will be overnight-fasted. This study will have a single-blind, randomized, crossover, repeated-measures experimental design. In each trial, after warm-up, the eccentric peak torque of the knee joint extensors will be determined using an isokinetic dynamometer. After this, participants will complete an isokinetic exercise until exhaustion at a constant angular velocity of 60° • s-1. After the exhaustion protocol, athletes will perform again the peak torque determination. Immediately after, participants will drink the P-CHO supplement or MS during the first 2 h. Both drinks will contain 0.8-1.2 g carbohydrates • kg-1 • h-1 and 0.2-0.4 g protein • kg-1 • h-1. Twenty four and 48 h after the exhaustion protocol participants will return to the laboratory to repeat the peak torque determination. Blood samples will be collected before warming-up, immediately and 2 h after the last peak torque determination and 24 h and 48 h after. Serum samples will be analyzed for creatine kinase, lactate dehydrogenase, interleukine-6, protein carbonyls and total antioxidant status. The delayed onset muscle soreness, using a visual analogue scale, and girths will be measured at the same moments as blood sampling. Two-way repeated-measures ANOVA will be used for statistical analysis of the data.
|Muscle Damage Inflammation Oxidative Stress Functional Recovery||Dietary Supplement: P-CHO supplement Dietary Supplement: Fruit Milk Shake|
|Study Design:||Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single Blind (Participant)
|Official Title:||Compared Effect of a Fruit Milk Shake With a Protein-Carbohydrate Supplement on Muscle Damage, Inflammation, Oxidative Stress and Functional Recovery, After Resistance Exercise|
- Maximal peak torque of the knee extensors muscles [ Time Frame: Up to 48 h ]To monitor functional recovery
- Creatine kinase [ Time Frame: Up to 48 h ]To monitor muscle damage
- Lactate dehydrogenase [ Time Frame: Up to 48 h ]To monitor muscle damage
- Interleukine-6 [ Time Frame: Up to 48 h ]To monitor the inflammatory process
- Total antioxidant status [ Time Frame: Up to 48 h ]To monitor oxidative stress
- Delayed onset muscle soreness [ Time Frame: Up to 48 h ]By a visual analogue scale, 10 cm in length. To monitor muscle soreness
- Mid-thigh girth, Three other girths will be measured: 5, 15 and 25 cm above the superior border of the patella. [ Time Frame: Up to 48 h ]To monitor the inflammatory process
- Protein carbonyls [ Time Frame: Up to 48 h ]To monitor oxidative stress
- Percentage of body fat [ Time Frame: 1 week before the first trial ]
- Food records [ Time Frame: 2 days before each trial ]To assess energy intake (kcal)
- Physical activity records [ Time Frame: 2 days before each trial ]To assess energy expenditure (kcal)
|Study Start Date:||March 2012|
|Study Completion Date:||May 2012|
|Primary Completion Date:||March 2012 (Final data collection date for primary outcome measure)|
Experimental: P-CHO supplement
This group will drink the P-CHO supplement in the first trial and the fruit milk shake in the second.
Dietary Supplement: P-CHO supplement
In the first trial, this group will drink a protein (P)-carbohydrate (CHO) supplement and in the second trial the fruit milk shake.
Other Name: Available in a common local market.
Experimental: Fruit Milk Sake
This group will drink the fruit milk shake in the first trial and the P-CHO supplement in the second.
Dietary Supplement: Fruit Milk Shake
The fruit content of the milk shake will be 100 g of strawberry and the amount of banana necessary to guaranty 0.8-1.2 g CHO • kg-1 BW • h-1.
Other Name: Strawberry, bananas, skimmed milk
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Please refer to this study by its ClinicalTrials.gov identifier: NCT01555775
|Faculdade de Desporto, Universidade do Porto|
|Porto, Portugal, 4200-450|
|Study Director:||José Soares, PhD||Faculdade de Desporto, Universidade do Porto|
|Principal Investigator:||Monica V. Sousa, BSc||Faculdade de Desporto, Universidade do Porto|
|Study Chair:||Vitor H Teixeira, PhD||Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto|