Donating Oral Mucosal Cells for the Study of ex Vivo Culture of Cells Sheets
Cultivated oral mucosal epithelial sheets on amniotic membrane have been reported to have good clinical results in treating patients with ocular surface disorders in both human and animal studies. However, such technique has not been well established in this hospital. The main purpose of this study is to obtain the discarded oral mucosal tissue from healthy donors who volunteer to donate their oral mucosa from buccal area. The obtained oral mucosal tissue will be cultured under different types (co-culture with or without 3T3 cells)。Inverted light microscopy, electron microscopy and immunostaining with K3/K12, K14, Ki67, P63, connexin 43, and involucrin will be preformed to test the effects of foreskin cell line co-culture system on cell condition. The investigators plan to start the study from Jan. 1st, 2011 to Dec. 31th, 2015. Thirty patients will be enrolled.
|Study Design:||Observational Model: Case-Only|
Donating oral mucosal cells for the study of ex vivo culture of cells sheets.
|Study Start Date:||January 2012|
|Estimated Study Completion Date:||December 2015|
|Estimated Primary Completion Date:||December 2015 (Final data collection date for primary outcome measure)|
Procedure: Donating oral mucosal cells
Donating oral mucosal cells
Please refer to this study by its ClinicalTrials.gov identifier: NCT01548261
|Contact: Wei Li Chen, MD, PhD||886-2-23123456 ext firstname.lastname@example.org|
|National Taiwan University Hospital, department of Ophthalmology||Recruiting|
|Taipei, Taiwan, 100|
|Contact: Wei Li Chen, MD, PhD 886-2-23123456 ext 65206 email@example.com|