Studying Biomarkers in Samples From Younger Patients With Wilms Tumor
RATIONALE: Studying samples of tissue and urine from patients with cancer in the laboratory may help doctor learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research trial studies biomarkers in samples from younger patients with Wilms tumor.
Genetic: gene expression analysis
Genetic: proteomic profiling
Genetic: reverse transcriptase-polymerase chain reaction
Genetic: western blotting
Other: immunohistochemistry staining method
Other: laboratory biomarker analysis
|Study Design:||Observational Model: Case-Only
Time Perspective: Retrospective
|Official Title:||Biological Analysis of Ethnic Variations in Wilms Tumor|
- Molecular "finger print" of primary Wilms tumor in ethnically diverse children [ Designated as safety issue: No ]
- Protein "finger print" from the urine of ethnically diverse children [ Designated as safety issue: No ]
|Study Start Date:||February 2012|
|Primary Completion Date:||May 2016 (Final data collection date for primary outcome measure)|
- To determine a unique molecular "finger print" of primary Wilms tumor arising in ethnically diverse children who are at increased risk to develop this lethal malignancy and subsequently relapse.
- To determine a unique protein "finger print" from the urine of ethnically diverse children who develop Wilms tumor and subsequent relapse.
OUTLINE: Formalin-fixed paraffin-embedded and snap-frozen primary tissue samples, and urine samples collected at the time of diagnosis and before treatment, are analyzed for molecular and protein profile by MALDI-TOF mass spectroscopy, western blotting, immunohistochemistry, and reverse-transcriptase polymerase chain reaction (RT-PCR) assays.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01542333
|Principal Investigator:||Harold N. Lovvorn, III, MD||Vanderbilt Children's Hospital|