Role of Biomarkers in Disease Progression in Samples From Patients With Acute Myeloid Leukemia
RATIONALE: Studying samples of blood, tissue, and bone marrow from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research trial studies the role of biomarkers in disease progression in samples from patients with acute myeloid leukemia.
Genetic: gene expression analysis
Genetic: gene rearrangement analysis
Genetic: polymerase chain reaction
Genetic: protein analysis
Genetic: western blotting
Other: laboratory biomarker analysis
Other: mass spectrometry
|Study Design:||Observational Model: Cohort
Time Perspective: Retrospective
|Official Title:||The Role of AF1q in Progression of Leukemia|
- Subcellular localization of the MLL-AF1q fusion protein in leukemia cells [ Designated as safety issue: No ]
|Study Start Date:||December 2011|
|Estimated Primary Completion Date:||January 2100 (Final data collection date for primary outcome measure)|
- To determine the subcellular localization of the MLL-AF1q fusion protein in leukemia cells.
OUTLINE: Cryopreserved specimens are analyzed for MLL-AF1q fusion protein and AF1 gene expression by mass spectrometry, western blot analysis, semi-quantitative PCR, and qPCR.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01500499
|Principal Investigator:||Julie D. Saba, MD, PhD||Children's Hospital & Research Center Oakland|