The Metabolite Profiling of Obese Subjects Before and After Sleeve Gastrectomy
|Study Design:||Observational Model: Case-Only
Time Perspective: Prospective
|Official Title:||The Impact of Sleeve Gastrectomy Surgery in Obese Subjects on Metabolite Profiling|
|Study Start Date:||July 2011|
|Study Completion Date:||June 2016|
|Primary Completion Date:||December 2012 (Final data collection date for primary outcome measure)|
In the present study we examine the metabolic fluctuations of the most important amino acids and fatty acids to better understand the metabolic process in weight reduction in obese subjects that underwent sleeve gastrectomy surgery.
The availability of the tandem equipment for amino acids and fatty acids determinations for the medical system gives us an opportunity to investigate the metabolic turmoil of weight reduction seen in obese subjects after sleeve gastrectomy.
We evaluated the lipid metabolism by the measurements of the fraction of carnitine fatty acids which is the most available one because only a small blood specimen from a finger puncture is needed.
In the present study we measured the blood concentrations of monocarboxylic and dicarboxylic fatty acids bounded to carnitine, free carnitine and also free amino acids 2 weeks before the surgery while subjects eat their regular high caloric diet, one week, one month and 3 month after the procedure.
Carnitne fatty acids comprise only the tip of the iceberg of all circulating fatty acids in the blood with a roughly distribution of: triglycerides, phospholipids and cholesteryl fatty acids 4 (Lipids43-65), free fatty acids 0.6, and carnitne fatty acids 0.005 mM. Some additional amount is also circulating in lipoproteins. Even carnitine monocarboxylic fatty acids comprise around 1% of the free fatty acids and around 0.1% of all fatty compounds, the fluctuations in their concentrations probably reflect the most dynamics metabolic events because fatty acid oxidation must initiate by binding fatty acid to carnitine. The concentration of dicarboxylic carnitine fatty acids is much higher and comprises about 2% for malic carnitine and about 10% for glutaric carnitine in the total amount of free acids (http://www.hmdb.ca/). Yet, as for monocarboxylic fatty acids, a remarkable mass of dicarboxylic acids is still present as free acids.
For all carnitne acyl compounds the distribution forms have a high variability on different physiological conditions.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01489657
|Wolfson Medical Center, Bariatric Surgery Clinic|
|Holon, Israel, 58100|
|Principal Investigator:||Zohar Landau, MD||Wolfson Medical Center|