Biomarkers in Tissue Samples From Patients With Ewing Sarcoma
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|ClinicalTrials.gov Identifier: NCT01480518|
Recruitment Status : Completed
First Posted : November 29, 2011
Last Update Posted : May 12, 2015
RATIONALE: Studying samples of tissue in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors understand how well patients respond to treatment.
PURPOSE: This research study is studying biomarkers in tissue samples from patients with Ewing sarcoma.
|Condition or disease||Intervention/treatment|
|Sarcoma||Genetic: DNA analysis Genetic: comparative genomic hybridization Genetic: gene expression analysis Genetic: mutation analysis Genetic: polymerase chain reaction Genetic: polymorphic microsatellite marker analysis Other: laboratory biomarker analysis|
- To describe the spectrum of GGAA-microsatellite polymorphisms at specific loci in genomic DNA prepared from Ewing sarcoma tumor specimens.
- To determine if there are differences in GGAA-microsatellite polymorphisms in genomic DNA prepared from Ewing sarcoma tumor specimens as compared to non-afflicted European and African normal genomic DNA.
- To determine if GGAA-microsatellite polymorphisms at specific loci in genomic DNA prepared from Ewing's sarcoma tumor specimens correlates with disease outcome in patients treated on COG protocol AEWS0031.
- To determine whether whole-genome amplification introduces alterations in GGAA-microsatellites as compared to non-amplified genomic DNA.
OUTLINE: Genomic PCR is used to amplify the microsatellites in the NR0B1 and GSTM4 promoters. In addition to determining microsatellite size, each microsatellite is sequenced following cloning into the pCR4 vector (Invitrogen) using standard topoisomerase cloning protocols.
|Study Type :||Observational|
|Actual Enrollment :||166 participants|
|Official Title:||Analysis of GGAA-Microsatellites in Ewing Sarcoma|
|Study Start Date :||December 2011|
|Actual Primary Completion Date :||January 2012|
|Actual Study Completion Date :||January 2012|
- Event-free survival
Biospecimen Retention: Samples With DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01480518
|Principal Investigator:||Stephen Lessnick, MD, PhD||University of Utah|