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ClinicalTrials.gov Identifier: NCT01438983
: September 22, 2011
Last Update Posted
: September 22, 2011
Shahid Sadoughi University of Medical Sciences and Health Services
Information provided by (Responsible Party):
Nastaran Azimi, Shahid Sadoughi University of Medical Sciences and Health Services
This is an observational, non-randomised trial to evaluate the effect of dietary type on infants oral candida colonization the investigators evaluated 90 infants in 1-24 months (45 breastfeeding and 45 bottle feeding infants) who attended to Yazd clinic for regular pediatric appointment. Infants with unremarkable health histories, normal weight, free of sign or symptom of oral candidiasis or other mucosal disease. Their mothers were also healthy and without sign or symptom of vagina or mammary candidiasis. Pacifier usage, use of antibiotics in last months, sign or symptom of oral candidiasis in infants or vagina and mammary candidiasis in mothers were exclusion criteria.
Condition or disease
Oral CandidaBreastfeedingBottle Feeding
After filling in a questionnaire about infants dietary intake, age and sex and their mothers education level and health condition, a pre-moistened sterile cotton swab was taken from dorsal surface of tongue and mid-plate. The swabs were cultured in agar plate containing sabourauds dextrose agar medium with chloramphenicol, and kept in 37 C for 48 hours. The number of candida colonies was counted and germ tube test performed to separate candida albicans from other types. The data was collected and analyzed by SPSS software and chi-square, logistic regression and mann-whitney test. Study hypothesis: feeding by mothers milk reduces oral candida colonization.
mean number of candida colonies [ Time Frame: 6 months ]
number of candida colonies growth in agar plate
Secondary Outcome Measures
prevalence of oral candida colonization in infants according to age and sex [ Time Frame: 6 months ]
exist of candida colony in agar plate
Biospecimen Retention: Samples With DNA
infants with unremarkable health histories, normal weight, free of sign or symptom of oral candidiasis or other mucosal disease.their mothers were also healthy and without sign or symptom of vagina or mammary candidiasis.
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Ages Eligible for Study:
1 Month to 24 Months (Child)
Sexes Eligible for Study:
Accepts Healthy Volunteers:
The investigators evaluated 90 infants in 1-24 months (45 breastfeeding and 45 bottle feeding infants) who attended to Yazd nikoopur clinic for regular pediatric appointment.