Protein Expression in Human Neuroblastoma Tumor Samples
RATIONALE: Studying protein expression in samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat neuroblastoma.
PURPOSE: This laboratory studying is looking at protein expression in tumor tissue samples from patients with neuroblastoma.
|Neuroblastoma||Genetic: protein expression analysis Genetic: western blotting Other: laboratory biomarker analysis|
|Study Design:||Observational Model: Case-Only
Time Perspective: Retrospective
|Official Title:||Analysis of Intersectin Expression in Primary Human Neuroblastomas|
- ITSN1 is highly expressed in neuroblastoma tumors and plays an important role in the development/progression of these tumors
|Study Start Date:||July 2011|
|Study Completion Date:||May 2016|
|Primary Completion Date:||May 2016 (Final data collection date for primary outcome measure)|
- To determine whether intersectin (ITSN1) proteins are present at elevated levels in primary human tumor samples.
OUTLINE: Archived tumor tissue samples are analyzed for intersectin (ITSN1) expression by western blot assays with enhanced chemiluminescence and exposed to X-ray film. The films are scanned, and levels of ITSN1 signal are quantified by densitometry.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01407731
|Principal Investigator:||John P. O'Bryan, PhD||University of Illinois at Chicago|