MyoGene: Effects of Acute Exercise on (Myokine) Gene Expression in Human Skeletal Muscle
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|ClinicalTrials.gov Identifier: NCT01316731|
Recruitment Status : Completed
First Posted : March 16, 2011
Last Update Posted : December 21, 2011
Rationale: Proteins released from muscle during and shortly after exercise, often referred to as myokines, may be central to our understanding of the cross-talk during and after exercise between skeletal muscles and other organs, in particular the liver. So far only a few myokines are identified (e.g. IL-6, IL-8, IL-15, TNF-alpha). Taking into account the role of these several known myokines in developing insulin resistance, revealing new putative myokines might provide valuable information and a direction for future research on the pathogenesis and treatment of type 2 diabetes mellitus.
Objective: The objective of the present study is to identify novel myokines, expression of which is altered in skeletal muscle after a single bout of exercise.
Study design: experimental study. Study population: Ten healthy, male subjects between 40 and 60 years of age and BMI < 30 kg/m2, will participate in this study.
Intervention: A single exercise bout that consists of one hour one-legged cycling on a adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as control for the exercising leg.
Main study outcomes: Main study outcomes include upregulation of genes in skeletal muscle after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels of selected proteins after exercise.
|Condition or disease||Intervention/treatment||Phase|
|Exercise Muscle Gene Expression Myokine||Other: Single legged exercise||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Actual Enrollment :||12 participants|
|Intervention Model:||Single Group Assignment|
|Masking:||None (Open Label)|
|Primary Purpose:||Basic Science|
|Official Title:||Effects of Acute Exercise on (Myokine) Gene Expression in Human Skeletal Muscle|
|Study Start Date :||February 2011|
|Actual Primary Completion Date :||June 2011|
|Actual Study Completion Date :||June 2011|
Other: Single legged exercise
A single exercise bout that consists of one hour one-legged cycling on a adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as control for the exercising leg.
- Gene expression changes in skeletal muscle from baseline to after exercise [ Time Frame: T = 0 and T =1 ]Gene expression is measured using whole genome Affymatrix microarrays Muscle tissue will be collected at time points T = 0 (before exercise) and T = 1 (directly after exercise). Special focus is on the changes in genes coding for myokines. Up to 5 genes will be selected for follow-up analysis.
- Changes in plasma levels of selected proteins [ Time Frame: T = 0, T =1 and T = 3 ]Selected proteins will be analyzed by ELISA assays or western blot analysis, depending on ELISA availability at T = 0, T = 1 and T = 3 (2 hours post-exercise). The selection of the proteins is based on significance, the robustness of induction (>80% of subjects showing induction) and the magnitude of the induction ( mean fold change > 2)
- PBMC gene expression changes before and after exercise [ Time Frame: T = 0, T = 1 and T =3 ]Gene expression changes in the blood will be assessed using whole genome Affymetrix microarrays for the samples at T = 0, 1 and 3.
- Routine plasma level changes before and after exercise [ Time Frame: T = 0, 1 and 3 ]At all three time point (T = 0, 1 and 3) plasma levels of glucose, insulin, fatty acids, triglycerides, cortisol, adrenalin and lactate will be determined.
- Heart rate changes, baseline compared with exercise [ Time Frame: During the intervention ]Heart rate will be measured during the maximum work load test and the experimental exercise period.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01316731
|Division of Human Nutrition, Wageningen University|
|Wageningen, Gelderland, Netherlands, 6703 HA|