Biomarkers in Samples of Bone Marrow From Patients With Acute Myeloid Leukemia
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|ClinicalTrials.gov Identifier: NCT01290107|
Recruitment Status : Completed
First Posted : February 4, 2011
Last Update Posted : May 18, 2016
RATIONALE: Studying samples of bone marrow from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is looking at bone marrow samples from patients with acute myeloid leukemia.
|Condition or disease||Intervention/treatment|
|Leukemia||Genetic: DNA analysis Genetic: gene rearrangement analysis Genetic: microarray analysis Genetic: polymerase chain reaction Other: immunological diagnostic method Other: laboratory biomarker analysis|
- To successfully transplant human acute myeloid leukemia (AML) cells into immunocompromised mice for the purpose of expansion of the cells.
- To harvest the cells and use chromatin immunoprecipitation (ChIP) methods to identify the locations of the protein complexes on the genome.
- To study the interactions of the Super Elongation Complex (SEC) and Dot1 Complex (DotCom) complexes in human leukemia samples.
- To compare the genomic targets of the complexes formed by MLL-ENL chimeras to non-MLL-rearranged leukemia samples to normal controls.
OUTLINE: Cryopreserved cells are implanted into NOD SCID gamma mice, expanded, harvested, and studied by chromatin immunoprecipitation (ChIP) methods (using antibodies to ENL, Af9, and AF10). Results are then analyzed by PCR, DNA sequencing, and/or microarray analysis.
|Study Type :||Observational|
|Estimated Enrollment :||9 participants|
|Official Title:||MLL Rearrangements in Acute Myeloid Leukemia: A Pilot Project to Expand and Study MLL-ENL in NOD SCID Gamma Mice|
|Study Start Date :||February 2011|
|Actual Primary Completion Date :||January 2015|
|Actual Study Completion Date :||May 2016|
- Successful transplantation of human acute myeloid leukemia (AML) cells into immunocompromised mice for the purpose of expansion of the cells
- Identification of the locations of the protein complexes
- Interactions of the SEC and DotCom complexes in human leukemia samples
Biospecimen Retention: Samples With DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01290107
|Principal Investigator:||Eric Guest, MD||Children's Mercy Hospital|