We updated the design of this site on September 25th. Learn more.
Show more
ClinicalTrials.gov
ClinicalTrials.gov Menu

Effect of Acute Red Raspberry Consumption on Post-prandial Oxidative Stress

This study has been completed.
Sponsor:
ClinicalTrials.gov Identifier:
NCT01242033
First Posted: November 16, 2010
Last Update Posted: December 6, 2010
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
Collaborator:
St. Michael's Hospital, Toronto
Information provided by:
University of Toronto
  Purpose
Eight healthy adult subjects will be given a meal of one, two or four cups red raspberries, or two slices white bread with or without 200 mg vitamin C, after an overnight fast and consumption of a low polyphenol diet for two days. Blood samples will be taken at various time points over an eight hour period to measure oxidative stress and antioxidant levels in the blood. Each subject will attend 5 study visits with one week intervals and be given each meal in a random order. It is hypothesized that raspberry consumption at higher doses will greater protect against meal-induced oxidative stress compared to bread controls.

Condition Intervention
Oxidative Stress Antioxidants Dietary Supplement: red raspberries Dietary Supplement: bread controls

Study Type: Interventional
Study Design: Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: An Acute Intervention Study Investigating the Effect of Red Raspberry Consumption on Post-prandial Oxidative Stress

Resource links provided by NLM:


Further study details as provided by University of Toronto:

Primary Outcome Measures:
  • change in antioxidant capacity from baseline (-0.25 h) [ Time Frame: -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake ]
    plasma antioxidant capacity measured using the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation assay and expressed in Trolox Equivalents (TE)


Secondary Outcome Measures:
  • change in lipid peroxidation from baseline (-0.25 h) [ Time Frame: -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake ]
    serum thiobarbituric acid reactive substances (TBARS) measured as an indicator of the lipid peroxidation product malondialdehyde (MDA)

  • change in thiols compared to baseline (-0.25 h) [ Time Frame: -025, 0.25, 0.5, 1, 2, 4 hours post-intake ]
    plasma thiols quantified by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) assay as an indicator of the level of protection against protein oxidation


Enrollment: 8
Study Start Date: February 2010
Study Completion Date: March 2010
Primary Completion Date: March 2010 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
Experimental: one cup red raspberries
treatment meal consists of one cup red raspberries
Dietary Supplement: red raspberries
single serving of one, two or four cups red raspberries
Experimental: two cups red raspberries
treatment meal consists of two cups red raspberries
Dietary Supplement: red raspberries
single serving of one, two or four cups red raspberries
Experimental: four cups red raspberries
treatment meal consists of four cups red raspberries
Dietary Supplement: red raspberries
single serving of one, two or four cups red raspberries
Placebo Comparator: bread
treatment meal consists of two slices white bread
Dietary Supplement: bread controls
two slices white bread alone or with 200 mg vitamin C supplement
Active Comparator: vitamin C
treatment meal consists of two slices white bread and 200 mg vitamin C in the form of supplemental ascorbic acid
Dietary Supplement: bread controls
two slices white bread alone or with 200 mg vitamin C supplement

  Eligibility

Information from the National Library of Medicine

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies.


Ages Eligible for Study:   20 Years to 30 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • healthy adults

Exclusion Criteria:

  • blood pressure greater than 120/80 mm Hg
  • body mass index greater than 24.9 kg/m^2
  • history of any chronic disease
  • currently taking medications
  • allergy to fruit
  Contacts and Locations
Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01242033


Locations
Canada, Ontario
Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital
Toronto, Ontario, Canada, M5C 2T2
Sponsors and Collaborators
University of Toronto
St. Michael's Hospital, Toronto
Investigators
Principal Investigator: A V Rao, PhD University of Toronto
  More Information

Responsible Party: Dr AV Rao, PhD, Professor Emeritus, Department of Nutritional Sciences, Faculty of Medicine, University of Toronto
ClinicalTrials.gov Identifier: NCT01242033     History of Changes
Other Study ID Numbers: 24774
First Submitted: November 8, 2010
First Posted: November 16, 2010
Last Update Posted: December 6, 2010
Last Verified: November 2010

Keywords provided by University of Toronto:
red raspberries
post-prandial
oxidative stress
antioxidants
polyphenols
vitamin C
acute dietary intervention

Additional relevant MeSH terms:
Vitamins
Ascorbic Acid
Micronutrients
Growth Substances
Physiological Effects of Drugs
Antioxidants
Molecular Mechanisms of Pharmacological Action
Protective Agents