Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men (METHYLHOMME)
This study will analyse the sperm global methylation status of 62 infertile men before assisted reproductive techniques. Some of these patients (20%) present hypomethylation of H19 locus. A global methylation analysis may reveal others imprinting defects.
|Study Design:||Observational Model: Case-Only
Time Perspective: Prospective
|Official Title:||Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men|
- Bring to light methylation abnormalities of the locus H19 in men's mature sperm cells presenting an unexplained oligozoospermia [ Time Frame: 1 day ] [ Designated as safety issue: No ]
- Determine if these methylation abnormalities of the locus H19 reflect changes in the profile of global methylation of the spermatic DNA [ Time Frame: 1 day ] [ Designated as safety issue: No ]
- Estimate the association between these modifications and the nuclear quality of the sperm cell [ Time Frame: 1 day ] [ Designated as safety issue: No ]by TUNEL analysis
- Estimate the association between these modifications and the rates of success with In VITRO fertilization [ Time Frame: 1 day ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples With DNA
Sample of sperm collected by automasturbation
|Study Start Date:||June 2009|
|Study Completion Date:||December 2012|
|Primary Completion Date:||September 2011 (Final data collection date for primary outcome measure)|
infertile patients presenting a reduced sperm count (less than 20 Millions of spermatozoa/ml)
Other: methylation analyses on spermatozoa from infertile men
Other Name: methylation analyses on spermatozoa from infertile men
An increase of the abnormalities of the imprint was brought back at the child's stemming from assisted reproductive techniques. Now abnormalities of methylation could be implied in defects of spermatogenesis and certain abnormalities of development of the male germ cells could be due to modifications abnormal epigenetics.
The objective of this research is to determine the frequency of arisen the abnormalities of methylation at the level of the locus H19 in the sperm cells of barren men presenting an unexplained oligozoospermia and to determine if these changes are a reflection of abnormalities of the profiles of methylation of the whole genome.
The patients will realize a taking of sperm having signed the consent.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01239186
|Department of Biology of reproduction (TENON Hospital)|
|Paris, Ile de France, France, 75020|
|Principal Investigator:||Célia Ravel, MD||TENON Hospital - APHP|