Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men (METHYLHOMME)
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|ClinicalTrials.gov Identifier: NCT01239186|
Recruitment Status : Completed
First Posted : November 11, 2010
Last Update Posted : August 7, 2013
|Condition or disease||Intervention/treatment|
|Oligospermia||Other: methylation analyses on spermatozoa from infertile men|
An increase of the abnormalities of the imprint was brought back at the child's stemming from assisted reproductive techniques. Now abnormalities of methylation could be implied in defects of spermatogenesis and certain abnormalities of development of the male germ cells could be due to modifications abnormal epigenetics.
The objective of this research is to determine the frequency of arisen the abnormalities of methylation at the level of the locus H19 in the sperm cells of barren men presenting an unexplained oligozoospermia and to determine if these changes are a reflection of abnormalities of the profiles of methylation of the whole genome.
The patients will realize a taking of sperm having signed the consent.
|Study Type :||Observational|
|Actual Enrollment :||49 participants|
|Official Title:||Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men|
|Study Start Date :||June 2009|
|Actual Primary Completion Date :||September 2011|
|Actual Study Completion Date :||December 2012|
infertile patients presenting a reduced sperm count (less than 20 Millions of spermatozoa/ml)
Other: methylation analyses on spermatozoa from infertile men
- Bring to light methylation abnormalities of the locus H19 in men's mature sperm cells presenting an unexplained oligozoospermia [ Time Frame: 1 day ]
- Determine if these methylation abnormalities of the locus H19 reflect changes in the profile of global methylation of the spermatic DNA [ Time Frame: 1 day ]
- Estimate the association between these modifications and the nuclear quality of the sperm cell [ Time Frame: 1 day ]by TUNEL analysis
- Estimate the association between these modifications and the rates of success with In VITRO fertilization [ Time Frame: 1 day ]
Biospecimen Retention: Samples With DNA
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Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01239186
|Department of Biology of reproduction (TENON Hospital)|
|Paris, Ile de France, France, 75020|
|Principal Investigator:||Célia Ravel, MD||TENON Hospital - APHP|