Evaluation of Discarded Laboratory Pathological Specimens and Media
The researchers propose to investigate the causes, incidence, and time-related events of chromosomal and physiologic abnormalities as they relate to patient diagnosis, fertility drugs utilized, and in vitro laboratory culture conditions.
Conditioned IVF Media
|Study Design:||Time Perspective: Prospective|
|Official Title:||Laboratory Evaluation of IVF Discarded Pathological Specimens and Media for Optimization of Techniques for Assisted Reproduction|
- Methods for the freezing of ooyctes and sperm [ Time Frame: duration of study ] [ Designated as safety issue: No ]To test, standardize and implement better methods for the freezing of ooyctes and sperm prior to these techniques being used in the clinical setting.
- New methods of gene and chromosome analysis [ Time Frame: duration of study ] [ Designated as safety issue: No ]The development of new methods to optimize the determination of genes and chromosomes in gametes.
Biospecimen Retention: Samples With DNA
Samples with DNA: Non-viable oocytes, spermatozoa, granulosa cells and follicular fluid Samples without DNA: seminal fluid, serum and conditioned IVF media
|Study Start Date:||July 2007|
|Estimated Study Completion Date:||December 2018|
|Estimated Primary Completion Date:||July 2017 (Final data collection date for primary outcome measure)|
We propose to identify a group of parameters that have significant predictive value for assisted reproductive technology outcomes. We seek to test, standardize and implement better methods for the freezing of oocytes and sperm prior to these techniques being used in the clinical setting. We seek to develop new methods to optimize the determination of genes and chromosomes in gametes. We hope to develop new cell surgery or micromanipulation techniques (e.g. use of cell drills and lasers in order to enhance the efficiency of procedures such as ICSI, assisted hatching, biopsy) as well as other manipulations. We will test the safety and efficiency of micro fluidics and automation in the Embryology lab. This has the potential to reduce cost, human errors, temperature and physical changes. We hope to develop new methods and media and media supplements normally found in the reproductive tract that allow for higher survival of gametes in vitro. We will also test the proficiency of laboratory staff members on techniques and/or procedures performed in the in-vitro fertilization laboratory. We will perform Quality Control to review how changes in temperature and setting can effect specimens
Please refer to this study by its ClinicalTrials.gov identifier: NCT01219439
|United States, New Jersey|
|Reproductive Medicine Assoicates of New Jersey|
|Morristown, New Jersey, United States, 09760|
|United States, Pennsylvania|
|Reproductive Medicine Associates of PA at Lehigh Valley|
|Allentown, Pennsylvania, United States, 18104|
|Principal Investigator:||Richard T Scott, MD||Reproductive Medicine Associates of New Jersey|