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Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia

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ClinicalTrials.gov Identifier: NCT01178463
Recruitment Status : Unknown
Verified August 2010 by Hillel Yaffe Medical Center.
Recruitment status was:  Active, not recruiting
First Posted : August 10, 2010
Last Update Posted : August 10, 2010
Sponsor:
Information provided by:
Hillel Yaffe Medical Center

Brief Summary:
The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).

Condition or disease Intervention/treatment
Male Infertility Other: Biospecimen staining

Study Type : Observational
Estimated Enrollment : 25 participants
Observational Model: Cohort
Time Perspective: Retrospective
Study Start Date : January 2010
Primary Completion Date : May 2010
Estimated Study Completion Date : October 2010


Group/Cohort Intervention/treatment
I. Obstructive Azoospermia Other: Biospecimen staining
The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies.
II. Non-Obstructive Azoospermia Patients Other: Biospecimen staining
The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies.



Primary Outcome Measures :
  1. SSCs were identified in both obstructive and nonobstructive azoospermia patients. [ Time Frame: One year ]


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Ages Eligible for Study:   18 Years and older   (Adult, Senior)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   No
Sampling Method:   Probability Sample
Study Population
Infertile male patients treated at the Hillel Yaffe Medical Center IVF Unit
Criteria

Inclusion Criteria:

  • Azoospermic patients

Exclusion Criteria:

  • Patients with sperm cells

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01178463


Locations
Israel
Hillel Yaffe Medical Center - IVF Unit
Hadera, Israel, 38100
Sponsors and Collaborators
Hillel Yaffe Medical Center

Responsible Party: Dr. Adrian Ellenbogen, Hillel Yaffe Medical Center
ClinicalTrials.gov Identifier: NCT01178463     History of Changes
Other Study ID Numbers: HYMC-0065-09
First Posted: August 10, 2010    Key Record Dates
Last Update Posted: August 10, 2010
Last Verified: August 2010

Additional relevant MeSH terms:
Infertility
Infertility, Male
Genital Diseases, Male
Genital Diseases, Female