Factors Involved in Obesity-related Inflammation and Insulin Resistance
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|ClinicalTrials.gov Identifier: NCT01173705|
Recruitment Status : Terminated
First Posted : August 2, 2010
Last Update Posted : January 20, 2017
In this project, we propose to recruit lean and obese subjects with different ethnic background (African Americans and Caucasians) to study the alterations of lipid and carbohydrate metabolism and determine whether these disturbances are linked to genetic, inflammatory, oxidative stress, and/or nutritional factors. Because systemic inflammation and insulin resistance are frequent features of obesity, we postulate that an unbalanced diet with high saturated- and low omega 3-fatty acids is linked to obesity-related inflammation and insulin resistance. We propose to investigate fatty acid metabolism and determine the links between fatty acid composition and oxidative stress in tissues of lean and obese subjects. We propose the following aims:
Specific Aim 1: Evaluate nutrient intake in lean and obese subjects using the standard NHANES Food Questionnaire.
Specific Aim 2: Evaluate the fatty acid composition, including omega-3, in adipose tissue depots, blood monocytes and skeletal muscle, and examine the relationship between omega-3 content and inflammatory and oxidative stress markers.
Specific Aim 3: Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.
|Condition or disease|
|Obesity Insulin Resistance Inflammation|
|Study Type :||Observational|
|Actual Enrollment :||62 participants|
|Official Title:||Genetic and Nutritional Factors Involved in Obesity-related Inflammation and Insulin Resistance|
|Study Start Date :||June 2011|
|Primary Completion Date :||January 2014|
|Study Completion Date :||January 2014|
Normal weight: abdominal surgery
Lean individuals undergoing elective abdominal surgery
Obese: abdominal or bariatic surgery
Obese subjects undergoing elective abdominal or bariatric surgery
- Examine the relationship between omega-3 content and inflammatory and oxidative stress markers [ Time Frame: 5 years ]We will evaluate fatty acid composition in adipose tissues depots, blood monocytes, and skeletal muscle to examine the relationship between omega-e content and inflammatory and oxidative stress markers.
- Evaluate nutrient intake [ Time Frame: 5 years ]Using the NHANES Food Questionnaire, we will evaluate nutrient intakes of lean and obese individuals
- Compare effects of omega-3 and saturated fatty acid supplementation on inflammatory and oxidative stress markers (in vitro) [ Time Frame: 5 years ]Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.
Biospecimen Retention: Samples With DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01173705
|United States, Tennessee|
|Vanderbilt University Medical Center|
|Nashville, Tennessee, United States, 37232|
|Principal Investigator:||Robyn Tamboli, PhD||Vanderbilt University Medical Center|
|Study Chair:||Naji N Abumrad, MD||Vanderbilt University Medical Center|