PD-1/PD-L1 Pathway Study on Septic Patients
The investigators want to identify that whether PD-1/PD-L1 pathway will change in human sepsis, and whether PD-1/PD-L1 pathway play an important role in sepsis induced immunosuppression.
|Study Design:||Observational Model: Case Control
Time Perspective: Cross-Sectional
|Official Title:||PD-1/PD-L1 Pathway in T Lymphocyte Apoptosis and Monocyte Dysfunction in Septic Patients|
peripheral venous blood from septic patients and healthy controls, anticoagulate with EDTA-K2
|Study Start Date:||December 2008|
|Study Completion Date:||April 2010|
|Primary Completion Date:||February 2010 (Final data collection date for primary outcome measure)|
First, we will detect PD-L1 expression on PBMC of septic patients and healthy controls.
Second, blockade of PD-1/PD-L1 pathway in vitro and stimulate with some biomolecules and then analyze the PBMC function and apoptosis by flow cytometry.
Then, compare with healthy controls and find the differences.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01161745
|Principal Investigator:||Xiao-ming Deng, doctor||Changhai Hospital, the Second Military Medical University|