Biomarker Changes in Samples From Young Patients With Acute Myeloid Leukemia
RATIONALE: Studying samples of blood or tumor tissue from patients with cancer in the laboratory may help doctors learn about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is studying biomarker changes in samples from young patients with acute myeloid leukemia.
Genetic: DNA methylation analysis
Genetic: gene expression analysis
Genetic: microarray analysis
Genetic: polymorphism analysis
Other: laboratory biomarker analysis
|Study Design:||Observational Model: Case-Only
Time Perspective: Retrospective
|Official Title:||Epigenetic Alterations in AML|
- Relationships between genetic and epigenetic landscapes in AML [ Designated as safety issue: No ]
|Study Start Date:||October 2009|
|Estimated Primary Completion Date:||January 2100 (Final data collection date for primary outcome measure)|
- Map key histone modifications and cytosine methylation in leukemia cell lines with defined oncogenic mutations.
- Acquire genome-wide maps of key histone modifications and cytosine methylation for primary leukemia in samples from patients with acute myeloid leukemia (AML).
- Investigate functional relationships between genetic and epigenetic landscapes in AML.
OUTLINE: Archived samples are analyzed in vivo and in vitro (cell line) for histone modification and cytosine methylation. Genome-wide locations of 5 histone modifications are analyzed using chip-Seq, as well as DNA methylation analysis at nucleotide-resolution by high-throughput bisulfite sequencing. These epigenetic data are correlated with genomic data (sequence analysis, expression array, SNP array).
Please refer to this study by its ClinicalTrials.gov identifier: NCT01139931
|Principal Investigator:||Soheil Meshinchi, MD||Fred Hutchinson Cancer Research Center|