Biomarkers in Samples From Young Patients With Acute Myeloid Leukemia
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about biomarkers related to cancer.
PURPOSE: This research study is studying biomarkers in samples from young patients with acute myeloid leukemia.
|Leukemia||Genetic: gene expression analysis Genetic: mutation analysis Other: flow cytometry Other: laboratory biomarker analysis|
|Study Design:||Observational Model: Case Control
Time Perspective: Retrospective
|Official Title:||Implications of s-SHIP Expression and SHIP Alterations in AML|
- Event-free survival
- Overall survival
- Relapse rate
Biospecimen Retention: Samples With DNA
|Study Start Date:||February 2013|
|Study Completion Date:||May 2016|
|Primary Completion Date:||May 2016 (Final data collection date for primary outcome measure)|
- Determine the extent and prognostic implications of variable s-SHIP expression and SHIP gene mutations in pediatric patients with acute myeloid leukemia.
- Determine the effects of aberrant s-SHIP expression on the PI3K/Akt pathway in these patients via electrochemiluminescence and phosphospecific flow cytometry assays.
OUTLINE: RNA samples are analyzed for variable s-SHIP expression and are screened for SHIP mutations. Cryopreserved cells (with known high- or low-s-SHIP expression) are analyzed for the levels of various components of the PI3K/Akt pathway via electrochemiluminescence and phosphospecific flow cytometry.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01138306
|Principal Investigator:||Phoenix Ho, MD||Fred Hutchinson Cancer Research Center|