Fat Perception in Humans (09-0873) (OT)
There are many substances naturally present in the mouth that may help us taste fat in food. Two of these substances (lipases and CD36) will be examined in this study.
The presence of fat in food increases food tastiness, therefore people often over-eat high-fat foods and gain weight. The purpose of this study is to determine if blocking lipases and some genetic variations in the CD36 gene will make fatty food less tasty so that people eat less. Our hypothesis is that Orlistat and a particular gene will increase one's ability to detect fat.
|Study Design:||Observational Model: Case Control
Time Perspective: Cross-Sectional
|Official Title:||Inhibition of Lipolysis in Oral Cavity and Fat Perception in Humans|
- Oleic Acid Detection Level [ Time Frame: Few weeks after screening ] [ Designated as safety issue: No ]We will measure oleic acid detection levels as a marker of subjects' ability to detect free fatty acids.
- Triolein Detection [ Time Frame: Few weeks after screening ] [ Designated as safety issue: No ]We will measure triolein detection levels as a marker of subjects' ability to detect triglyceride.
Biospecimen Retention: Samples With DNA
whole blood will be collected and frozen
|Study Start Date:||May 2010|
|Study Completion Date:||June 2014|
|Primary Completion Date:||June 2014 (Final data collection date for primary outcome measure)|
rs1761667- AA genotype
subjects carrying the CD36 genotype rs1761667, i.e. a Single Nucleotide Polymorphism that significantly reduces CD36 level and has a minor allele frequency of 38-48%.
subjects who are homozygous of CD36 genotype rs1761667-G allele.
Heterozygous of CD36 gene rs1761667-A genotype.
Determine the effect of lipolysis inhibition in oral cavity on orosensory detection of fat in humans.
Hypothesis: Orally applied Orlistat will increase detection thresholds of TAGs but not FFA in humans.
To test this hypothesis we will measure triolein (a TAG) and oleic acid (a FFA) taste detection thresholds with a three-alternative forced-choice (3-AFC) ascending concentration method under two conditions (Orlistat day vs. Control day). To control for olfactory input, subjects will be assessed wearing noseclips.
- Determine the effect of CD36 variants on fat taste perception in humans. Hypothesis: SNPs that associate with reduced CD36 expression will be associated with higher FFA and TAG detection thresholds
To test this hypothesis we will measure triolein and oleic acid taste detection thresholds in subjects who carry of the common CD36 e-SNP rs1761667 (i.e. a SNP that significantly reduces CD36 level and has a minor allele frequency of 38-48%). We expect subjects who are at least heterozygous for the expression reducing allele to have higher triolein and oleic acid taste detection thresholds compared to non-carrier subjects.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01128400
|United States, Missouri|
|Washington University School of Medicine|
|St. Louis, Missouri, United States, 63110|
|Principal Investigator:||Marta Y Pepino de Gruev, Ph.D.||Washington University School of Medicine|