Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia

This study has been completed.
Information provided by:
National Cancer Institute (NCI) Identifier:
First received: January 26, 2010
Last updated: June 5, 2010
Last verified: June 2010

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.

Condition Intervention
Genetic: RNA analysis
Genetic: gene expression analysis
Genetic: mutation analysis
Genetic: protein expression analysis
Other: fluorescence activated cell sorting
Other: laboratory biomarker analysis

Study Type: Observational
Official Title: Critical Role of MicroRNA-34a and MicroRNA-194 in Acute Myeloid Leukemia With CEBPA Mutations

Resource links provided by NLM:

Further study details as provided by National Cancer Institute (NCI):

Primary Outcome Measures:
  • Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis [ Designated as safety issue: No ]
  • Relationship between overexpression of microRNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations and granulocytic differentiation [ Designated as safety issue: No ]
  • Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis [ Designated as safety issue: No ]
  • Role of microRNA-34a and microRNA-194 in myeloid cell proliferation [ Designated as safety issue: No ]

Estimated Enrollment: 6
Study Start Date: January 2010
Primary Completion Date: June 2010 (Final data collection date for primary outcome measure)
Detailed Description:


  • To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
  • To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation.
  • To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
  • To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.

OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.


Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No


  • Diagnosis of acute myeloid leukemia with CEBPA mutations


  • Not specified


  • Not specified
  Contacts and Locations
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Please refer to this study by its identifier: NCT01057199

Sponsors and Collaborators
Children's Oncology Group
Principal Investigator: Soheil Meshinchi, MD Fred Hutchinson Cancer Research Center
  More Information

Additional Information:
No publications provided

Responsible Party: Gregory H. Reaman, Children's Oncology Group - Group Chair Office Identifier: NCT01057199     History of Changes
Other Study ID Numbers: CDR0000664229, COG-AAML10B13
Study First Received: January 26, 2010
Last Updated: June 5, 2010
Health Authority: United States: Federal Government

Keywords provided by National Cancer Institute (NCI):
adult acute myeloid leukemia
childhood acute myeloid leukemia/other myeloid malignancies

Additional relevant MeSH terms:
Leukemia, Myeloid
Leukemia, Myeloid, Acute
Neoplasms by Histologic Type processed this record on April 16, 2015