Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia
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|ClinicalTrials.gov Identifier: NCT01057199|
Recruitment Status : Completed
First Posted : January 27, 2010
Last Update Posted : May 7, 2015
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.
|Condition or disease||Intervention/treatment|
|Leukemia||Genetic: RNA analysis Genetic: gene expression analysis Genetic: mutation analysis Genetic: protein expression analysis Other: fluorescence activated cell sorting Other: laboratory biomarker analysis|
- To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
- To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation.
- To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
- To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.
OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.
|Study Type :||Observational|
|Actual Enrollment :||6 participants|
|Official Title:||Critical Role of MicroRNA-34a and MicroRNA-194 in Acute Myeloid Leukemia With CEBPA Mutations|
|Study Start Date :||January 2010|
|Actual Primary Completion Date :||June 2010|
|Actual Study Completion Date :||June 2010|
- Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis
- Relationship between overexpression of microRNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations and granulocytic differentiation
- Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis
- Role of microRNA-34a and microRNA-194 in myeloid cell proliferation
Biospecimen Retention: Samples With DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01057199
|Principal Investigator:||Soheil Meshinchi, MD||Fred Hutchinson Cancer Research Center|