Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia
|ClinicalTrials.gov Identifier: NCT01057199|
Recruitment Status : Completed
First Posted : January 27, 2010
Last Update Posted : May 7, 2015
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.
|Condition or disease||Intervention/treatment|
|Leukemia||Genetic: RNA analysis Genetic: gene expression analysis Genetic: mutation analysis Genetic: protein expression analysis Other: fluorescence activated cell sorting Other: laboratory biomarker analysis|
- To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
- To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation.
- To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
- To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.
OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.
|Study Type :||Observational|
|Actual Enrollment :||6 participants|
|Official Title:||Critical Role of MicroRNA-34a and MicroRNA-194 in Acute Myeloid Leukemia With CEBPA Mutations|
|Study Start Date :||January 2010|
|Primary Completion Date :||June 2010|
|Study Completion Date :||June 2010|
- Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis
- Relationship between overexpression of microRNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations and granulocytic differentiation
- Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis
- Role of microRNA-34a and microRNA-194 in myeloid cell proliferation
Biospecimen Retention: Samples With DNA
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01057199
|Principal Investigator:||Soheil Meshinchi, MD||Fred Hutchinson Cancer Research Center|