Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome (IBS)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT01028898
Recruitment Status : Unknown
Verified December 2006 by Chinese PLA General Hospital.
Recruitment status was:  Active, not recruiting
First Posted : December 9, 2009
Last Update Posted : February 11, 2011
Information provided by:
Chinese PLA General Hospital

Brief Summary:
In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Condition or disease
Irritable Bowel Syndrome Digestive System Diseases Gastrointestinal Diseases Intestinal Diseases Colonic Diseases Colonic Diseases, Functional

Detailed Description:
For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Study Type : Observational
Estimated Enrollment : 180 participants
Observational Model: Case Control
Time Perspective: Prospective
Official Title: Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.
Study Start Date : January 2006
Estimated Primary Completion Date : August 2011
Estimated Study Completion Date : December 2012

Biospecimen Retention:   Samples With DNA
The investigators enrolled IBS-D and IBS-C patients on the Rome III criteria and healthy volunteers as normal control group. Biopsies were taken from the cecum and sigmoid colon under direct vision. Five pieces were taken separately at each site. Four pieces were processed by cold saline water and frozen in liquid nitrogen immediately while the other one was fixed in formalin and then embedded in paraffin three days later.

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Ages Eligible for Study:   18 Years to 60 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Probability Sample
Study Population
IBS patients (IBS-D and IBS-C)and healthy control

Inclusion Criteria:

  • Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

Exclusion Criteria:

  • Patients with organic illnesses or a history of major abdominal surgery
  • Major psychotic episode, mental retardation or dementia
  • Pregnant women and lactational women
  • Postinfectious IBS, PI-IBS

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT01028898

Sponsors and Collaborators
Chinese PLA General Hospital
Study Chair: YANG Yun-sheng, MD Chinese PLA General Hospital
Principal Investigator: ZHANG Chun-yan, doctor Chinese PLA General Hospital
Principal Investigator: WANG Weifeng, doctor Chinese PLA General Hospital
Principal Investigator: YAO XIN, DOCTOR Chinese PLA General Hospital
Principal Investigator: PENG LIHUA, DOCTOR Chinese PLA General Hospital
Principal Investigator: YU YUANZI, DOCTOR Chinese PLA General Hospital

Publications automatically indexed to this study by Identifier (NCT Number):
Responsible Party: YANG Yun-sheng, ChinaPLAGH Identifier: NCT01028898     History of Changes
Other Study ID Numbers: IBS and metabolism
First Posted: December 9, 2009    Key Record Dates
Last Update Posted: February 11, 2011
Last Verified: December 2006

Keywords provided by Chinese PLA General Hospital:
fluorescent quantitation polymerase chain reaction
Western blot
immunohistochemistry staining (IHC)
ATP content

Additional relevant MeSH terms:
Irritable Bowel Syndrome
Gastrointestinal Diseases
Digestive System Diseases
Colonic Diseases
Intestinal Diseases
Colonic Diseases, Functional
Pathologic Processes