Study of Tissue and Blood Samples From Patients With Low-Grade Glioma
RATIONALE: Studying samples of tumor tissue and blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors predict how patients will respond to treatment.
PURPOSE: This research study is looking at tissue and blood samples from patients with low-grade glioma.
Brain and Central Nervous System Tumors
Genetic: fluorescence in situ hybridization
Genetic: loss of heterozygosity analysis
Genetic: polymerase chain reaction
Other: flow cytometry
Other: immunohistochemistry staining method
|Study Design:||Observational Model: Case-Only
Time Perspective: Retrospective
|Official Title:||Diagnostic and Prognostic Markers in Low-Grade Gliomas|
- Alterations in chromosomes 7, 9p, 10, 17, 19q, X, or Y as determined by FISH [ Time Frame: baseline ] [ Designated as safety issue: No ]
- Loss of chromosomal materials in chromosomes 9p, 10, 13, 17, or 22 by PCR analysis [ Time Frame: baseline ] [ Designated as safety issue: No ]
- Levels of PCNA, Ki-67 (MIB-1), or mutated p53 by immunostaining with monoclonal antibodies [ Time Frame: baseline ] [ Designated as safety issue: No ]
- DNA ploidy by FISH and flow cytometry [ Time Frame: baseline ] [ Designated as safety issue: No ]
- Percentage of cells in S-phase or G2M-phase as measured by flow cytometry [ Time Frame: baseline ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples With DNA
|Study Start Date:||December 1995|
|Study Completion Date:||March 2013|
|Primary Completion Date:||March 2013 (Final data collection date for primary outcome measure)|
Previously preserved paraffin-embedded tissue blocks are obtained and used for biomarker studies. Blood samples obtained during treatment are also obtained. Loss of heterozygosity of specific chromosomal regions are performed using PCR analysis of microsatellite repeats (41,118-120) on DNA extracted from the paraffin-embedded archival specimens. FISH and flow cytometry may also be used to assess chromosomal loss of deletion. Immunohistochemistry is also performed.
|Genetic: fluorescence in situ hybridization Genetic: loss of heterozygosity analysis Genetic: polymerase chain reaction Other: flow cytometry Other: immunohistochemistry staining method|
- Evaluate the diagnostic and prognostic relevance of alterations of specific chromosomes and chromosomal regions including 7, 9p, 10p, 10q, 13q, 17p, 17q, 19q, 22q, X, and Y, using PCR analysis of microsatellite repeats and FISH.
- Evaluate the diagnostic and prognostic relevance of DNA ploidy by flow cytometric analysis; compare with ploidy determination by FISH.
- Assess the diagnostic and prognostic relevance of various markers of cellular proliferation and cellular function including flow cytometric determination of %S-phase, %G2M, and immunohistochemical evaluation of PCNA, Ki-67, and p53.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01004523
Show 38 Study Locations
|Study Chair:||Jan Buckner, MD||Mayo Clinic|