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Release of Cytokines by Stimulated Peripheral Blood Mononuclear Cells (PBMC) From Chronic Periodontitis Subjects

This study has been completed.
Information provided by:
Escola Bahiana de Medicina e Saude Publica Identifier:
First received: October 5, 2009
Last updated: NA
Last verified: October 2009
History: No changes posted

Background and Objective: It has been reported that peripheral blood mononuclear cells (PBMC) from periodontal patients have a different profile of cytokine release when compared to the ones of healthy patients. Cigarette smoking is a recognized risk factor for periodontitis and is known to affect the systemic and local immune responses. Thus, the aim of the present study was to obtain preliminary data about the interaction of periodontal condition and smoking in the secretion of some cytokines by PBMC.

Material and Methods: PBMC samples were isolated from 19 donors, divided into generalized chronic periodontitis (P; n=10) and periodontal health (H; n=9) groups. In addition, the smokers and non-smokers of each group were separately evaluated: SP- smokers with severe generalized chronic periodontitis (n=5); SH- periodontally healthy smokers (n=5); NSP- non-smokers with severe generalized chronic periodontitis (n=5) and NSH- periodontally healthy non-smokers (n=4). Cells were incubated for 24-48 hours in 500 µL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of TNF-α, IL-6, IL-8 and IL-10 released using ELISA kits.


Study Type: Observational
Study Design: Observational Model: Case Control
Time Perspective: Cross-Sectional

Further study details as provided by Escola Bahiana de Medicina e Saude Publica:



Ages Eligible for Study:   25 Years to 55 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
healthy humans

Inclusion Criteria:

  • periodontitis

Exclusion Criteria:

  • diabetes
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Please refer to this study by its identifier: NCT00990041

Faculdade de Odontologia de Piracicaba
Piracicaba, SP, Brazil, 13000
Sponsors and Collaborators
Escola Bahiana de Medicina e Saude Publica
  More Information Identifier: NCT00990041     History of Changes
Other Study ID Numbers: 9002-10-04.PBMC
Study First Received: October 5, 2009
Last Updated: October 5, 2009

Keywords provided by Escola Bahiana de Medicina e Saude Publica:
find cytokines in blood of periodontal patients

Additional relevant MeSH terms:
Chronic Periodontitis
Periodontal Diseases
Mouth Diseases
Stomatognathic Diseases processed this record on September 25, 2017