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Study of the Effect of Eicosanoids on Contractile Activity of Pregnant Human Myometrium in Pathological Situation (EAU2)

The recruitment status of this study is unknown. The completion date has passed and the status has not been verified in more than two years.
Verified March 2015 by Université de Sherbrooke.
Recruitment status was:  Recruiting
Fonds de la Recherche en Santé du Québec
Information provided by (Responsible Party):
Jean-Charles Pasquier, MD, PhD, Université de Sherbrooke Identifier:
First received: July 14, 2009
Last updated: March 3, 2015
Last verified: March 2015

Recent studies show that EET and 20-HETE have important biological effects, particularly in the vascular system. The investigators studied the effect of eicosanoids on the gravid rat uterus. The results suggest that 20-HETE had an tocolytics effect on gravid uterus. In the previous study, we demonstrated that the enzymes of the pathway of EET were present in human uterine tissues. Moreover, the addition of an inhibitor of degradation of EET had an tocolytic effect on the human myometrium, as the exogenous addition of 8.9, 14,15-EET and 20-HETE.


Primary objective: To compare the balance of different metabolic pathways of arachidonic acid (AA) of the pregnant human myometrium in pathological situations (preterm labor, uterine atony, prolonged pregnancy).

Specific objectives: i) To study the effect of derived from the AA on in vitro contractile activity of normal and pathological uterine tissues, and ii) detect and quantify the different sub-products of metabolism of AA in the uterine tissues (myometrium, fetal membranes and placenta).

The management of uterine contraction is in the heart of modern obstetrics year, yet the progress made in other specialties, based on the study of smooth muscle have not yet been transposed in obstetrics. A better understanding of systems for regulating the contraction is important in terms of 1) new physiological knowledge, but it could also be the source of 2) modification of strategies to take care of premature delivery (new Tocolytic), or 3) improving the efficiency of uterine muscle during delivery or 4) for treatment of patients with prolonged pregnancy.

Obstetric Labor Complications
Prolonged Pregnancy

Study Type: Observational
Study Design: Observational Model: Case-Only
Time Perspective: Prospective
Official Title: Study of the Effect of Eicosanoids on Contractile Activity of Pregnant Human Myometrium in Pathological Situation.

Further study details as provided by Université de Sherbrooke:

Primary Outcome Measures:
  • effect of eicosanoids on contractile activity of myometrium of pregnant women with pathological situations [ Time Frame: during c-section ]

Secondary Outcome Measures:
  • effect of enzymatic inhibitors on contractile activity of myometrium from pregnant women with pathological situations [ Time Frame: during c-section ]
  • detection of enzymes from the different pathways [ Time Frame: after c-section ]
  • quantification of eicosanoids in different tissues [ Time Frame: after c-section ]

Biospecimen Retention:   Samples Without DNA
uterus biopsy

Estimated Enrollment: 130
Study Start Date: May 2009
Estimated Study Completion Date: October 2015
Estimated Primary Completion Date: July 2015 (Final data collection date for primary outcome measure)
Women who will have a c-section at the CHUS

Detailed Description:

It is a clinical study with a slope fundamental aims to examine the metabolic pathways of AA of human myometrium and their functional roles according to their clinical profile divided into three contractile pathological situations - threat of premature delivery, dynamic dystocia, prolonged pregnancy - and two groups of patients at term: a group before work and group work.

The sampling method. After birth, a biopsy will be perform from the lower segment of the uterus. After caesarean sections of membrane and placenta are collected.

The substances studied during isometric tension tests are part of the three degradation pathways of the AA.

  1. new eicosanoids in cumulative dose (8,9-EET, 11,12-EET, 14,15-EET, 20-HETE), and in combination
  2. enzyme inhibitors of the eicosanoids pathway (AUDA, MS-PPOH, DDMS), and the COX and LOX pathways (indomethacin), alone or in combination.

Ages Eligible for Study:   18 Years to 40 Years   (Adult)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
women who will have a c-section at the CHUS

Inclusion Criteria:

  • all women who will have a c-section

Exclusion Criteria:

  • induction of labor,
  • child with malformation,
  • birth weight less than 2500 grams or greater than 4500g
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Please refer to this study by its identifier: NCT00939744

Contact: Stéphanie Corriveau, BSc 819 346-1110 ext 13875
Contact: Simon Blouin, PhD 819 346-1110 ext 13877

Canada, Quebec
Centre hospitalier de l'Université de Sherbrooke Recruiting
Sherbrooke, Quebec, Canada, J1H 5N4
Sponsors and Collaborators
Université de Sherbrooke
Fonds de la Recherche en Santé du Québec
Principal Investigator: Jean-Charles Pasquier, MD, PhD Centre hospitalier de l'Université de Sherbrooke
Principal Investigator: Rousseau Éric, PhD Université de Sherbrooke
  More Information

Publications automatically indexed to this study by Identifier (NCT Number):
Responsible Party: Jean-Charles Pasquier, MD, PhD, Dr, Université de Sherbrooke Identifier: NCT00939744     History of Changes
Other Study ID Numbers: 09-040
Study First Received: July 14, 2009
Last Updated: March 3, 2015

Keywords provided by Université de Sherbrooke:
contractile activity
pregnant women
obstetrics complications

Additional relevant MeSH terms:
Obstetric Labor Complications
Pregnancy, Prolonged
Pregnancy Complications processed this record on May 23, 2017