Studying Tissue Samples to Learn More About Drug Resistance in Patients With Acute Myeloid Leukemia
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about cancer and the development of drug resistance in patients.
PURPOSE: This laboratory study is examining tissue samples from patients with acute myeloid leukemia to learn more about drug resistance in these patients.
|Leukemia||Other: flow cytometry Other: immunological diagnostic method Other: laboratory biomarker analysis|
|Study Design:||Observational Model: Other
Time Perspective: Retrospective
|Official Title:||Comparison of P-gp Efflux and Accumulation Assay Results With ECOG 3999 Leukemia Samples|
- Percentage of specimens collected from patients that are found to be P-glycoprotein (P-gp)-positive by accumulation assay that were found to be negative using efflux assay [ Time Frame: 1 day ]
|Actual Study Start Date:||March 28, 2006|
|Study Completion Date:||December 30, 2013|
|Primary Completion Date:||December 30, 2013 (Final data collection date for primary outcome measure)|
- Ascertain whether assessments of P-glycoprotein (P-gp) status using the accumulation assay find greater than 10% positive specimens among 30 specimens (collected from patients with acute myeloid leukemia enrolled on clinical trial ECOG-E3999) found to be negative using only the efflux assay.
OUTLINE: This is a multicenter study.
Cryopreserved bone marrow specimens are examined for P-glycoprotein (P-gp) by the accumulation assay and the efflux assay using DiOC_2 dye. Flow cytometry is used for measuring activity in both assays.
PROJECTED ACCRUAL: A total of 40 specimens will be accrued for this study.
Please refer to this study by its ClinicalTrials.gov identifier: NCT00900380
|Study Chair:||Elisabeth Paietta, PhD||Our Lady of Mercy Medical Center|