Studying T Cells in Blood and Bone Marrow Samples From Patients With Multiple Myeloma
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|ClinicalTrials.gov Identifier: NCT00897910|
Recruitment Status : Terminated (Funding unavailable)
First Posted : May 12, 2009
Results First Posted : December 12, 2013
Last Update Posted : January 11, 2016
RATIONALE: Studying samples of blood and bone marrow from patients with cancer in the laboratory may help doctors learn more about T cells and plan better treatment for multiple myeloma.
PURPOSE: This research study is looking at T cells in blood and bone marrow samples from patients with multiple myeloma.
|Condition or disease||Intervention/treatment|
|Multiple Myeloma and Plasma Cell Neoplasm||Other: flow cytometry Other: immunoenzyme technique Other: laboratory biomarker analysis|
- To evaluate the feasibility of expanding myeloma-specific T cells using autologous ex vivo expanded B cells loaded with myeloma antigens as antigen-presenting cells (B-APCs) in peripheral blood and bone marrow samples from patients with multiple myeloma.
- To examine the feasibility of selecting and expanding myeloma-specific T cells ex vivo using interferon γ release and CD3/CD28 stimulation.
OUTLINE: Peripheral blood and bone marrow samples are collected periodically for laboratory studies. Samples are analyzed to assess the feasibility of expanding autologous B cells ex vivo using CD40L and IL-4; the antigen-presenting phenotype of autologous B-cell antigen-presenting cells (B-APCs) using flow cytometry; and the antigen-presenting function of B-APCs using ELISPOT and chromium-release assay. Myeloma-specific interferon γ secreting T cells are isolated and selected using Miltenyi beads. The selected myeloma-specific T cells are expanded ex vivo using anti CD3/CD28 beads.
|Study Type :||Observational|
|Actual Enrollment :||6 participants|
|Official Title:||Strategies to Isolate and Expand Myeloma Specific T-cells Using Autologous B Cells as Antigen Presenting Cell B-APC|
|Study Start Date :||April 2008|
|Actual Primary Completion Date :||November 2009|
|Actual Study Completion Date :||September 2012|
Other: flow cytometry
- Percentage of Myeloma-specific T Cells ex Vivo Expanded Using Flow Cytometry [ Time Frame: Collection of PBMCs over a period of 9-12 months, and the laboratory component will be performed over another year. ]
- Cell Counts of Myeloma-specific T Cells ex Vivo Expanded Before and After CD3/CD28 Stimulation [ Time Frame: Collection of PBMCs over a period of 9-12 months, and the laboratory component will be performed over another year. ]
Biospecimen Retention: Samples Without DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00897910
|United States, Michigan|
|Barbara Ann Karmanos Cancer Institute|
|Detroit, Michigan, United States, 48201-1379|
|Principal Investigator:||Zaid Al-Kadhimi, MD||Barbara Ann Karmanos Cancer Institute|