Phase II Study of Digitoxin to Treat Cystic Fibrosis

This study is ongoing, but not recruiting participants.
Information provided by (Responsible Party):
Pamela L. Zeitlin, MD, PhD, Johns Hopkins University Identifier:
First received: October 29, 2008
Last updated: September 16, 2015
Last verified: September 2015

This study will measure the inflammatory effects of digitoxin on IL-8 and neutrophil counts in induced sputum in stable Cystic Fibrosis (CF) patients and the pharmacokinetics of digitoxin in serum.

Funding Source-FDA OOPD

Condition Intervention Phase
Cystic Fibrosis
Drug: digitoxin
Other: placebo
Phase 2

Study Type: Interventional
Study Design: Allocation: Randomized
Endpoint Classification: Safety/Efficacy Study
Intervention Model: Parallel Assignment
Masking: Double Blind (Subject, Caregiver, Investigator)
Primary Purpose: Treatment
Official Title: Phase II Study of Digitoxin to Treat Cystic Fibrosis

Resource links provided by NLM:

Further study details as provided by Johns Hopkins University:

Primary Outcome Measures:
  • To measure the effects of digitoxin on IL-8 and neutrophil counts in induced sputum in stable CF patients. [ Time Frame: Day 0 to Day 42 ] [ Designated as safety issue: No ]

Secondary Outcome Measures:
  • To measure the pharmacokinetics of digitoxin in serum in stable CF patients. [ Time Frame: PK on Day 0, sputum Days 0, 14, 21, 28 and 42 ] [ Designated as safety issue: No ]
  • To measure safety indices, including ECG changes and sputum microbiology, in stable CF patients. [ Time Frame: ECG on Days 0 through Day 42, sputum microbiology at Screening and Day 42 ] [ Designated as safety issue: Yes ]
  • To measure quality of life scores using the CFQ-R [ Time Frame: Days 0, 14, 28 and 42 ] [ Designated as safety issue: No ]
  • To measure the effect of digitoxin on gene expression in nasal epithelial cells of stable CF patients [ Time Frame: Day 0, Day 28 ] [ Designated as safety issue: No ]
    Rhinoprobe to collect nasal epithelial cells.

Estimated Enrollment: 24
Study Start Date: August 2010
Estimated Study Completion Date: February 2016
Primary Completion Date: June 2014 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
Active Comparator: 1
low dose 0.05mg digitoxin given once daily for 28 days
Drug: digitoxin
0.05mg tabs, once daily for 28 days
Active Comparator: 2
higher dose 0.1mg digitoxin daily for 28 days
Drug: digitoxin
0.1mg pills, once daily for 28 days.
Placebo Comparator: 3
placebo given daily for 28 days
Other: placebo
pill taken once daily for 28 days

Detailed Description:
The study will be conducted as a randomized, double blind, placebo-controlled, repeat dosing trial evaluating the effects of 28 days of digitoxin on IL-8 and neutrophil concentrations in induced sputum in subjects with mild to moderate cystic fibrosis lung disease. Twenty-four total patients will be randomized into 3 groups of 8 subjects each (0.05 mg or 0.1 mg digitoxin or a placebo).

Ages Eligible for Study:   18 Years to 45 Years
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No

Inclusion Criteria

  • Male or female 18-45 years of age
  • Confirmed diagnosis of CF based on the following criteria:positive sweat chloride > or = 60 mEq/liter (by pilocarpine iontophoresis) and/or a genotype with two identifiable mutations consistent with CF
  • FEV1 > or = 40% predicted value at screening
  • Weight > 45 kg at Screening and Visit 1 (dosing)
  • Clinically stable with no evidence of acute upper or lower respiratory tract infection or current pulmonary exacerbation (see Appendix II) or treatment of a pulmonary exacerbation within the 14 days prior to Screen Visit. Subject may rescreen 14 days after they complete treatment for a pulmonary exacerbation, if healthy at that time.
  • Ability to perform Spirometry.
  • Ability to understand and sign a written informed consent and comply with the requirements of the study.

Exclusion Criteria:

  • Use of an investigational agent within the 4-week period prior to Screen visit.
  • Use of a medication with anti-neutrophil or anti-inflammatory effect or those known to have an effect on inflammatory outcomes [azithromycin, gentamicin, amikacin, colistin, ibuprofen, celecoxib, or other NSAIDs, prednisone or other corticosteroids(systemic or inhaled), such as Advair, cromolyn (Intal®), montelukast (Singulair®), zafirlukast (Accolate®), zileuton (Zyflo®), and any immunosuppressive agent within the 4 weeks prior to Visit #1, Day 1 and until their participation in the study ends (after Visit 6). See NOTE at end of exclusionary criteria for subjects on oral antibiotic therapy.
  • Use of topical nasal steroid products for at least 2 weeks prior to study drug administration and discontinued use until after the nasal cell collection at Day 28.
  • Inability or unwillingness to stop macrolide antibiotics 4 weeks prior to Day 1 until their participation in the study ends. Prior use of macrolide antibiotics, including those for maintenance therapy will not exclude the subject from participation.
  • History of significant cardiac disease or cardiac arrhythmia
  • Presence of an arrhythmia identified on screening ECG or 24 hour holter monitor
  • Pulmonary hypertension
  • History of significant cardiac disease or cardiac arrhythmia
  • Presence of a clinically significant arrhythmia identified on screening ECG or 24 hour holter monitor.
  • Pulmonary hypertension
  • Burkholderia species in sputum within 2 years or at Screen visit
  • Drugs known to interact with digitoxin including phenobarbital, amphotericin B, rifampicin, diltiazem, and verapamil or drugs that would potentiate potassium loss (certain diuretics or excessive laxative use, defined as more than twice daily use of miralax).
  • Unwillingness to use beta-agonists (or levalbuterol) prior to induced sputum procedures.
  • Oxygen saturation < 92% on room air at Screen visit
  • Pregnant, breastfeeding, or unwilling to use an effective form of birth control for the duration of the study
  • History of significant hemoptysis > or = 60cc per episode during the 30 days prior to Screening visit
  • Significant history of hepatic, cardiovascular, renal, neurological, hematologic, or peptic ulcer disease
  • SGOT (ALT) or SGPT (AST) > 3 times the upper limit of normal at Screen, documented biliary cirrhosis, or portal hypertension
  • Creatinine > 1.8 mg/dL at Screen
  • Inability to swallow pills
  • Potassium, serum <3.3 mEq/L at screening
  • Known inability to produce sputum (if unable to expectorate, must be able to produce an induced sputum sample at screening).
  • Presence of a condition or abnormality that in the opinion of the Investigator would compromise the safety of the subject or the quality of the data NOTE: For subjects on continuous antibiotic therapy for at least 6 months one continuous antibiotic or alternating two different antibiotics, they can maintain their current therapy. If the subject is alternating between two different inhaled antibiotics each month, Visit 1 should coincide with the "on" cycle of one of the inhaled antibiotics for consistency during the treatment period. For subjects on alternate month TOBI®, colistin or Cayston therapy, the "off" cycle must coincide with the Treatment Phase of the study. Subjects should be scheduled for Screening Visit during their one-month "on" period, and may resume taking TOBI®, colistin or Cayston after completion of Visit 6 (Day 42) or early termination.
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Please refer to this study by its identifier: NCT00782288

United States, Maryland
Johns Hopkins Hospital
Baltimore, Maryland, United States, 21287
Sponsors and Collaborators
Pamela L. Zeitlin, MD, PhD
Principal Investigator: Pamela L Zeitlin, MD, PhD Johns Hopkins University, School of Medicine, Pediatric Pulmonary
  More Information

Additional Information:
No publications provided

Responsible Party: Pamela L. Zeitlin, MD, PhD, Professor of Pediatrics, Johns Hopkins University Identifier: NCT00782288     History of Changes
Other Study ID Numbers: FD-R-003456-01
Study First Received: October 29, 2008
Last Updated: September 16, 2015
Health Authority: United States: Food and Drug Administration

Keywords provided by Johns Hopkins University:
inflammatory markers
gene expression

Additional relevant MeSH terms:
Cystic Fibrosis
Digestive System Diseases
Genetic Diseases, Inborn
Infant, Newborn, Diseases
Lung Diseases
Pancreatic Diseases
Pathologic Processes
Respiratory Tract Diseases
Anti-Arrhythmia Agents
Cardiotonic Agents
Cardiovascular Agents
Enzyme Inhibitors
Molecular Mechanisms of Pharmacological Action
Pharmacologic Actions
Physiological Effects of Drugs
Protective Agents
Therapeutic Uses processed this record on November 25, 2015