Generation of Dendritic Cell Precursors From Cord Blood Stem Cells
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|ClinicalTrials.gov Identifier: NCT00731744|
Recruitment Status : Unknown
Verified August 2008 by National Taiwan University Hospital.
Recruitment status was: Recruiting
First Posted : August 11, 2008
Last Update Posted : August 11, 2008
There is a growing interest in generating dendritic cells (DCs) for using as vaccines. Several cytokines, especially stem cell factor (SCF) and FLT3-ligand (FL), have been identified as essential to produce large numbers of myeloid precursors and even to increase DC yield obtained by the action of granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor alpha (TNF-α). However, there are few studies on the effect of the early-acting cytokines, commonly used to expand CD34+ progenitor cells, on DC generation.
We have established a serious of functional analysis of maturated or immaturated DCs including surface markers screening or cytokine production. Here, we design this two-year project to evaluate the possible methodology in the generation of CD14+CD1a- and CD14- CD1a+ myeloid DC precursors from CD34+ cells. In the first year of the project, we will set up the standard protocol to generate DC precursors from cord blood stem cells. In the first step, we will isolate CD34+ cells from cord blood and amplify these cells. Purity of the CD34+ cell populations obtained will be greater than 90%. In the next step, these primary cultures of CD34+ cells will treat with early-acting cytokines such as GM-CSF and IL-4 to further induction into dendritic cells. Functional analysis of dendritic cells would be performed in this stage. In the second year of the project, we will further evaluate the induction of DCs by CD34+ stem cells could be affected by combination of mesenchymal stromal cells. These results of this research with CD34+-based DCs induction provide the basis for materials to develop the DC-based vaccine against viral and fungal infections, and might be a powerful tool in anti-tumor immunotherapy.
|Condition or disease||Intervention/treatment||Phase|
|Normal Full-Term Deliveries||Procedure: Normal full-term deliveries||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Estimated Enrollment :||50 participants|
|Intervention Model:||Single Group Assignment|
|Masking:||None (Open Label)|
|Primary Purpose:||Basic Science|
|Official Title:||Generation of Dendritic Cell Precursors From Cord Blood Stem Cells|
|Study Start Date :||January 2007|
|Actual Primary Completion Date :||February 2007|
|Estimated Study Completion Date :||December 2010|
- Procedure: Normal full-term deliveries
Normal full-term deliveries by standard procedures according to institutional guidelines
- None, we isolate stem cells from cord blood. [ Time Frame: from CS operation to close ]
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00731744
|Contact: Chien-Nan Lee, Associate Professor||886-2-23123456 ext firstname.lastname@example.org|
|National Taiwan University Hospital||Recruiting|
|Taipei, Taiwan, 100|
|Contact: Chien-Nan Lee, ASSOCIATE PROFESSOR 886-2-23123456 ext 5166 email@example.com|