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Corneal Epithelial Wound Healing Difference Between Human Autoserum and Cord Blood Serum

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
ClinicalTrials.gov Identifier: NCT00681642
Recruitment Status : Completed
First Posted : May 21, 2008
Last Update Posted : January 10, 2011
Sponsor:
Information provided by:
National Taiwan University Hospital

Brief Summary:
Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.

Condition or disease
Corneal Epithelial Wound Healing

Study Type : Observational
Actual Enrollment : 7 participants
Observational Model: Case Control
Time Perspective: Prospective
Official Title: Phase 1 Study of Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Autoserum and Cord Blood Serum—in Vitro Cell Culture Experiment
Study Start Date : March 2007
Primary Completion Date : March 2008
Study Completion Date : March 2008

Resource links provided by the National Library of Medicine

U.S. FDA Resources

Group/Cohort
1
Corneal epithelial tissue with wound cultured in human autoserum
2
Corneal epithelial tissue with wound cultured in umbilical cord serum



Primary Outcome Measures :
  1. Cell proliferation [ Time Frame: 1 week ]

Secondary Outcome Measures :
  1. Cell migration [ Time Frame: 1 week ]


Information from the National Library of Medicine

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Ages Eligible for Study:   Child, Adult, Senior
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
Healthy pregnant women underwent smooth cesarean section during labor Healthy blood donor
Criteria

Inclusion Criteria:

  • Healthy pregnant women
  • Cesarean section for labor
  • Complete placenta with umbilical cord retained
  • Healthy individual

Exclusion Criteria:

  • The retained placenta and umbilical cord were not complete
  • Individual with anemia or other hematologic disorder unsuitable for blood donation

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00681642


Locations
Taiwan
National Taiwan University Hospital, department of Ophthalmology
Taipei, Taiwan, 10047
Sponsors and Collaborators
National Taiwan University Hospital
Investigators
Principal Investigator: Wei-Li Chen, MD, PhD National Taiwan University Hospital, department of Ophthalmology

Publications:

Responsible Party: Wei-Li Chen, MD, PhD, Assistant Professor, National Taiwan University Hospital, department of Ophthalmology
ClinicalTrials.gov Identifier: NCT00681642     History of Changes
Other Study ID Numbers: 200702037R
First Posted: May 21, 2008    Key Record Dates
Last Update Posted: January 10, 2011
Last Verified: December 2010

Keywords provided by National Taiwan University Hospital:
Corneal epithelial wound
Human autoserum
Cord blood

Additional relevant MeSH terms:
Wounds and Injuries