Acute Effect of Fructose on Lipid Metabolism and Gender Differences

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00620360
Recruitment Status : Completed
First Posted : February 21, 2008
Last Update Posted : February 24, 2012
Information provided by (Responsible Party):
Luc Tappy, MD, University of Lausanne

Brief Summary:

It has been widely documented that fructose overfeeding increases plasma triglycerides and hepatic de novo lipogenesis, and impairs insulin sensitivity in healthy male volunteers. The effect of gender on the metabolic responses to fructose remains an important open question, however.

The objective of this study is to compare the effect of an acute oral fructose load on carbohydrate and lipid metabolism in healthy young males and females.

Condition or disease Intervention/treatment Phase
Lipid Metabolism Dietary Supplement: fructose Dietary Supplement: Fructose Not Applicable

Detailed Description:

The study is aimed at comparing the effects of oral fructose on several specific metabolic pathways in males and females.Participants will receive an isoenergetic diet containing 55% carbohydrate, 15% protein and 35% lipids for three days prior to testing. After this period of controlled diet, they will be studied for 2 hours in the post-absorptive state (Time 0-120 min) and over a 6 hours period (Time 120-480 min) during which they will receive 4 loads of 0,30 g/kg fat free mass U-13C labelled fructose, at times 120, 180, 240, 300. Throughout the study, deuterated glucose and glycerol will be infused to monitor whole body glucose production and glycerol turnover.

The following parameters will be monitored in basal conditions and after the ingestion of the load of fructose:

  • Glycerol turnover(glycerol 2H5)
  • de novo lipogenesis (incorporation of 13C into palmitate of VLDLs)
  • whole body energy expenditure and net substrate oxydation (indirect calorimetry)
  • net fructose oxidation (breath 13CO2)
  • glucose turnover: (6,6 2H2 Glucose)
  • plasma glucose, free fatty acids, ketone bodies, lactate, insulin, triacylglycerol, total cholesterol, VLDL, LDL, and HDL subfractions

An adipose tissue (periumbilical subcutaneous) biopsy will be obtaine by needle aspiration under local anesthesia in fasting conditions (time 0 min) and after fructose (time 480 min) to assess the effects of fructose on adipose gene expression profile. Key genes involved in the regulation of carbohydrate (GLUT 4, hexokinase, PDH-kinase), lipid (FAT-CD36, FABP, acetylCoA carboxylase, malonyl-CoA decarboxylase, PPARg) and energy metabolism (PGC-1a, UCP2)will be monitored

Results obtained in males and females will be compared with two-way analysis of variance

Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 18 participants
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: Acute Effect of Fructose on Lipid Metabolism and Gender Differences
Study Start Date : January 2008
Actual Primary Completion Date : December 2008
Actual Study Completion Date : December 2008

Resource links provided by the National Library of Medicine

Drug Information available for: Fructose
U.S. FDA Resources

Arm Intervention/treatment
Experimental: fructose
acute fructose administration
Dietary Supplement: fructose
acute administration of 4 times 0.3g fructose/kg lean body mass
Dietary Supplement: Fructose
acute administration of 4 times 0.3 g/kg fat-free mass oral fructose

Primary Outcome Measures :
  1. Hepatic de novo lipogenesis [ Time Frame: acute effect of dietary fructose (within 6 hours) ]

Secondary Outcome Measures :
  1. Whole body lipid oxidation, glucose turnover, glycerol turnover, plasma substrates, hormone and energy expenditure (free fatty acid, glucose, lactate, beta-hydroxybutyrate, glycerol, VLDL- Triglycerides and insulin) expression of key adipose genes [ Time Frame: acute effect of fructose (within 6 hours) ]

Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 35 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Males and females
  • Age 18-35
  • Healthy
  • Body mass indexes (BMI) between 19 and 25 kg/m2
  • Informed consent obtained

Exclusion Criteria:

  • Smokers
  • Alcohol intake > 30g/day
  • Drug abuse
  • Diabetes mellitus

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00620360

Centre Hospitalier Universitaire Vaudois
Lausanne, Vaud, Switzerland, 1005
Sponsors and Collaborators
University of Lausanne
Principal Investigator: Luc Tappy, MD University of Lausanne

Responsible Party: Luc Tappy, MD, Professor of physiology, University of Lausanne Identifier: NCT00620360     History of Changes
Other Study ID Numbers: 279/07/CE/FBM
First Posted: February 21, 2008    Key Record Dates
Last Update Posted: February 24, 2012
Last Verified: February 2012

Keywords provided by Luc Tappy, MD, University of Lausanne:
dietary fructose
plasma lipids
healthy humans
gender differences
hepatic de novo lipogenesis