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Effects of Dietary Proteins on Hepatic Lipid Metabolism

This study has been completed.
University Hospital Inselspital, Berne
Information provided by:
University of Lausanne Identifier:
First received: November 14, 2007
Last updated: February 9, 2012
Last verified: November 2009

Individuals submitted to a high-fat or a high-fructose/sucrose diet develop, over a 6 day-period, several features of the metabolic syndrome, including increased plasma triglycerides, increased intrahepatic lipids, and decreased hepatic insulin sensitivity. It has been recently observed that the increase in intrahepatic lipids observed after a high fat diet is largely prevented when protein intake is concomitantly increased. This suggests that dietary protein protects the liver against some of the deleterious effects of a high fat diet. Mechanisms underlying this effect of protein may include an increased hepatic fat oxidation.

The aims of this study are:

  1. to evaluate the effects of dietary protein on several major pathways involved in hepatic lipid metabolism ( ketogenesis, lipid oxidation, de novo lipogenesis, VLDL-triglyceride secretion
  2. to determine whether the decrease in intra-hepatic lipids observed when dietary protein intake is increased are to be attributed to acute or long-term effects of proteins

Condition Intervention
Metabolic Syndrome X Other: high protein intake

Study Type: Observational
Study Design: Time Perspective: Prospective
Official Title: Effects of Dietary Proteins on Hepatic Lipid Metabolism

Resource links provided by NLM:

Further study details as provided by University of Lausanne:

Primary Outcome Measures:
  • Whole body lipid oxidation Medium chain triglyceride oxidation Long chain triglyceride oxidation VLDL-triglyceride kinetics Hepatic de novo lipogenesis whole body glucose and glycerol turnover [ Time Frame: acute effects of dietary proteins and after 4 days on a high protein day ]

Secondary Outcome Measures:
  • Energy expenditure Glucagon/insulin ratio Plasma growth hormone concentrations gene expression in subcutaneous adipose tissue Plasma ketone bodies concentrations [ Time Frame: acute effects of dietary proteins and after 4 days on a high protein day ]

Enrollment: 22
Study Start Date: January 2009
Study Completion Date: April 2009
Primary Completion Date: April 2009 (Final data collection date for primary outcome measure)
Groups/Cohorts Assigned Interventions
healthy volunteers Other: high protein intake
acute high protein intake chronic (6-day) high protein intake acute+chronic high protein intake control

  Show Detailed Description


Ages Eligible for Study:   18 Years to 30 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
Healthy volunteers

Inclusion Criteria:

  • age 18-30
  • sex male
  • BMI 19-25 kg/m2
  • sedentary
  • good physical health

Exclusion Criteria:

  • family history of diabetes
  • use of drugs or illicit substances
  • consumption of alcohol >50 g/week
  • vegetarians
  • smokers
  Contacts and Locations
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Please refer to this study by its identifier: NCT00558740

Centre Hospitalier Universitaire Vaudois
Lausanne, Vaud, Switzerland, CH-1011
Sponsors and Collaborators
University of Lausanne
University Hospital Inselspital, Berne
Principal Investigator: l Tappy, MD University of Lausanne
  More Information

Responsible Party: Prof. L uc Tappy, Department of Physiology, University of Lausanne Identifier: NCT00558740     History of Changes
Other Study ID Numbers: protocol 66/07/CE/FBM
SNF 310000-109737
Study First Received: November 14, 2007
Last Updated: February 9, 2012

Keywords provided by University of Lausanne:
Dietary protein
Hepatic de novo lipogenesis
Lipid oxidation

Additional relevant MeSH terms:
Metabolic Syndrome X
Insulin Resistance
Glucose Metabolism Disorders
Metabolic Diseases processed this record on August 17, 2017