Effects of Dietary Proteins on Hepatic Lipid Metabolism

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00558740
Recruitment Status : Completed
First Posted : November 15, 2007
Last Update Posted : February 10, 2012
University Hospital Inselspital, Berne
Information provided by:
University of Lausanne

Brief Summary:

Individuals submitted to a high-fat or a high-fructose/sucrose diet develop, over a 6 day-period, several features of the metabolic syndrome, including increased plasma triglycerides, increased intrahepatic lipids, and decreased hepatic insulin sensitivity. It has been recently observed that the increase in intrahepatic lipids observed after a high fat diet is largely prevented when protein intake is concomitantly increased. This suggests that dietary protein protects the liver against some of the deleterious effects of a high fat diet. Mechanisms underlying this effect of protein may include an increased hepatic fat oxidation.

The aims of this study are:

  1. to evaluate the effects of dietary protein on several major pathways involved in hepatic lipid metabolism ( ketogenesis, lipid oxidation, de novo lipogenesis, VLDL-triglyceride secretion
  2. to determine whether the decrease in intra-hepatic lipids observed when dietary protein intake is increased are to be attributed to acute or long-term effects of proteins

Condition or disease Intervention/treatment
Metabolic Syndrome X Other: high protein intake

  Show Detailed Description

Study Type : Observational
Actual Enrollment : 22 participants
Time Perspective: Prospective
Official Title: Effects of Dietary Proteins on Hepatic Lipid Metabolism
Study Start Date : January 2009
Actual Primary Completion Date : April 2009
Actual Study Completion Date : April 2009

Group/Cohort Intervention/treatment
healthy volunteers Other: high protein intake
acute high protein intake chronic (6-day) high protein intake acute+chronic high protein intake control

Primary Outcome Measures :
  1. Whole body lipid oxidation Medium chain triglyceride oxidation Long chain triglyceride oxidation VLDL-triglyceride kinetics Hepatic de novo lipogenesis whole body glucose and glycerol turnover [ Time Frame: acute effects of dietary proteins and after 4 days on a high protein day ]

Secondary Outcome Measures :
  1. Energy expenditure Glucagon/insulin ratio Plasma growth hormone concentrations gene expression in subcutaneous adipose tissue Plasma ketone bodies concentrations [ Time Frame: acute effects of dietary proteins and after 4 days on a high protein day ]

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Ages Eligible for Study:   18 Years to 30 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
Healthy volunteers

Inclusion Criteria:

  • age 18-30
  • sex male
  • BMI 19-25 kg/m2
  • sedentary
  • good physical health

Exclusion Criteria:

  • family history of diabetes
  • use of drugs or illicit substances
  • consumption of alcohol >50 g/week
  • vegetarians
  • smokers

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00558740

Centre Hospitalier Universitaire Vaudois
Lausanne, Vaud, Switzerland, CH-1011
Sponsors and Collaborators
University of Lausanne
University Hospital Inselspital, Berne
Principal Investigator: l Tappy, MD University of Lausanne

Responsible Party: Prof. L uc Tappy, Department of Physiology, University of Lausanne Identifier: NCT00558740     History of Changes
Other Study ID Numbers: protocol 66/07/CE/FBM
SNF 310000-109737
First Posted: November 15, 2007    Key Record Dates
Last Update Posted: February 10, 2012
Last Verified: November 2009

Keywords provided by University of Lausanne:
Dietary protein
Hepatic de novo lipogenesis
Lipid oxidation

Additional relevant MeSH terms:
Metabolic Syndrome X
Insulin Resistance
Glucose Metabolism Disorders
Metabolic Diseases