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The Cytogenetic Constitution of Embryos Resulting From Immature Oocytes (M1)

This study has been completed.
Information provided by:
Assaf-Harofeh Medical Center Identifier:
First received: October 14, 2007
Last updated: October 15, 2007
Last verified: September 2005

To examine the chromosomal number content in the blastomeres of embryos achieved from fertilization of immature oocytes at MI stage recruited following COH for IVF.

comparison of the genetic number content of embryos and their quality in terms of cleavage rate and morphological score reached after fertilization of immature oocytes at MI stage whether arrested at the MI stage or extruded the polar body, after in vitro maturation for a variable length of time.


Study Type: Observational
Study Design: Time Perspective: Prospective

Resource links provided by NLM:

Further study details as provided by Assaf-Harofeh Medical Center:

Enrollment: 23907
Study Start Date: October 2005
Study Completion Date: March 2007
Detailed Description:
Patients undergoing routine IVF, following informed consent, donated some of their unusable, immature- MI stage oocytes for our research. Following in vitro maturation for up to 2, 4, 8 and 24 hours after harvest, all immature oocytes in these 4 study groups underwent intracytoplasmic donor sperm injection (ICSI), whether they extruded their polar body (MI-PB or rescued MI) or not (arrested MI). Oocyte fertilization rate after 24 hours and embryonic development in terms of cleavage rate and morphology were assessed, for another 48 hours. Then the whole embryo was prepared for molecular cytogenetic evaluation using FISH (Fluorescence in situ hybridization) analysis of chromosomes x, y and 18.

Ages Eligible for Study:   25 Years to 35 Years   (Adult)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Patients with presence of immature oocytes after ART stimulation

Exclusion Criteria:

  • Patients with less than total 7 oocytes
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Please refer to this study by its identifier: NCT00544531

Infertility unit,Assaf-Harofeh Medical Center
Zrifin, Israel, 70300
Sponsors and Collaborators
Assaf-Harofeh Medical Center
Principal Investigator: Raphael Ron-el, professor Assaf-Harofeh Medical Center
  More Information Identifier: NCT00544531     History of Changes
Other Study ID Numbers: 76/05
Study First Received: October 14, 2007
Last Updated: October 15, 2007

Keywords provided by Assaf-Harofeh Medical Center:
MI oocytes, FISH,chromosomal aberrations

Additional relevant MeSH terms:
Genital Diseases, Male
Genital Diseases, Female processed this record on June 23, 2017