Chromosomal and Morphological Structure of Mature and Immature Male Germ Cells and Recognition of IL-18 in Testicular Tissue of Azoospermic Men

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00544089
Recruitment Status : Completed
First Posted : October 16, 2007
Last Update Posted : October 16, 2007
Information provided by:
Assaf-Harofeh Medical Center

Brief Summary:

To develop a biotechnological assay which will be able to recognize germ cells of the spermatogenic lineand and to identify the chromosomal and morphological structure of mature and immature male germ cells, in cases of of sperm production disorders(azoospermic patients).

To recognize IL-18 in testicular tissue of azoospermic men compared to control group as it has been suggested that this cytokine may serve as a paracrine defense factor in the male gonad associated to spermatogenesis.

Condition or disease

Detailed Description:
A new biotechnological assay was developed by adapting an existing computerized system which scans cell morphology and chromosomal ploidy in the same cell of hematologic line using digital technology, to recognize germ cells of the testis by their morphology and cytogenetics. Testicular tissues which obtained from 15 men were enzymatically digested and the cells received were fixed on slides. The cells were stained by Giemsa May Grinwald staining and scanned automatically in a bright field for cell morphology. Fluorescence in situ hybridization (FISH) using centromeric probes for chromosomes X, Y and 18 was performed on the same slide after destaining. The cells were automatically scanned and classified according to the ploidy of the chromosome signals. The presence of IL-18 in the same men's testis was examined parallelly, by immunohistochimical staining using specific antibody for human IL-18.

Study Type : Observational
Actual Enrollment : 25907 participants
Time Perspective: Prospective
Study Start Date : March 2006
Actual Study Completion Date : September 2007

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Infertility

Information from the National Library of Medicine

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Ages Eligible for Study:   22 Years to 39 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Non-obstructive azoospermic patients

Exclusion Criteria:

  • Obstructive azoospermia

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00544089

Infertility unit, Asaf-Harofeh Medical Center
Zrifin, Israel
Sponsors and Collaborators
Assaf-Harofeh Medical Center
Principal Investigator: Raphael Ron-el, professor Assaf-Harofeh Medical Canter Identifier: NCT00544089     History of Changes
Other Study ID Numbers: 37/06
First Posted: October 16, 2007    Key Record Dates
Last Update Posted: October 16, 2007
Last Verified: March 2006

Keywords provided by Assaf-Harofeh Medical Center:

Additional relevant MeSH terms:
Genital Diseases, Male
Genital Diseases, Female