This site became the new on June 19th. Learn more.
Show more Menu IMPORTANT: Listing of a study on this site does not reflect endorsement by the National Institutes of Health. Talk with a trusted healthcare professional before volunteering for a study. Read more... Menu IMPORTANT: Talk with a trusted healthcare professional before volunteering for a study. Read more... Menu
Give us feedback

A Search for Helicobacter Pylori in Localized Vulvodynia

This study has been completed.
Information provided by:
Western Galilee Hospital-Nahariya Identifier:
First received: July 13, 2007
Last updated: NA
Last verified: July 2007
History: No changes posted
Hypothesis: to examine a possible association between localized vulvodynia and H. pylori infection.

Vulvodynia Vulvar Vestibulitis

Study Type: Observational
Study Design: Observational Model: Case Control
Observational Model: Natural History
Time Perspective: Cross-Sectional
Time Perspective: Retrospective
Official Title: A Laboratory and Clinical Search for the Presence of H. Pylori and Treatment Against it in Localized Vulvodynia (Vestibulitis, Vestibulodynia).

Resource links provided by NLM:

Further study details as provided by Western Galilee Hospital-Nahariya:

Enrollment: 27
Study Start Date: May 2004
Study Completion Date: June 2007
Detailed Description:

Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa. Immunostaining for H. pylori was done as described. In short, tissue sections were deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in phosphate-buffered saline (PBS), and subjected to pretreatment with Proteinase K (8 minutes). The sections were quenched with 3% hydrogen peroxidase, incubated with protein block for 15 minutes at room temperature, and washed in PBS. Tissues then were incubated with polyclonal rabbit anti-H pylori antibody (dilution, 1:10; clone ch-20 429, DAKO, Carpinteria, CA). Finally, sections were washed in 0.05% polysorbate 20 in PBS, pH 7.4, and the bound antibody was detected using streptavidin and biotinylated secondary antibody with diaminobenzidine as the chromogen. Sections were counterstained with hematoxylin, dehydrated, and mounted. Negative controls were sections treated as above, but instead of incubation with the primary antibody, they were incubated with 1% bovine serum albumin in PBS.

Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls.

The positive and negative control gastric tissues for the immunohistochemical stain of the H. pylori microorganisms were obtained from the archives of the Department of Pathology.


Ages Eligible for Study:   18 Years to 50 Years   (Adult)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   No

Inclusion Criteria:

  • Paraffin blocks of vestibular tissues from patients with Vestibulodynia

Exclusion Criteria:

  • Patients without vestibulodynia
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its identifier: NCT00501774

Pathology Laboratory
Haifa, Israel
Sponsors and Collaborators
Western Galilee Hospital-Nahariya
Principal Investigator: Jacob Bornstein, MD westen Galilee Hospital
  More Information

Publications: Identifier: NCT00501774     History of Changes
Other Study ID Numbers: Sabo-1
Study First Received: July 13, 2007
Last Updated: July 13, 2007

Keywords provided by Western Galilee Hospital-Nahariya:
Localized vulvodynia
Helicobacter pylori
peptic disease

Additional relevant MeSH terms:
Vulvar Vestibulitis
Vulvar Diseases
Genital Diseases, Female
Vulvitis processed this record on September 19, 2017