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PTC124 for the Treatment of Cystic Fibrosis

This study has been completed.
Information provided by:
PTC Therapeutics Identifier:
First received: July 11, 2006
Last updated: October 16, 2007
Last verified: October 2007
In some patients with cystic fibrosis (CF), the disease is caused by a nonsense mutation (premature stop codon) in the gene that makes the cystic fibrosis transmembrane regulator (CFTR) protein. PTC124 has been shown to partially restore CFTR production in animals with CF due to a nonsense mutation. In an ongoing Phase 2a study being performed at the Hadassah University Hospital - Mount Scopus in Israel, patients with nonsense-mutation-mediated CF have received PTC124 in two 14-day treatment courses. Preliminary results from that study indicate that PTC124 has pharmacodynamic activity of PTC124 in CF and that PTC124 can be safely administered in this patient population. This Phase 2b extension study is designed to evaluate the activity and safety of 3 months (approximately 84 days) of continuous treatment with PTC124 in the same patients who were enrolled to the Phase 2a study. The main purpose of this study is to understand whether PTC124 can be safely administered and achieve pharmacodynamic activity in patients with CF due to a nonsense mutation.

Condition Intervention Phase
Cystic Fibrosis
Drug: PTC124
Phase 2

Study Type: Interventional
Study Design: Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Open Label
Primary Purpose: Treatment
Official Title: A Phase 2b Extension Study of PTC124 as an Oral Treatment for Nonsense-Mutation-Mediated Cystic Fibrosis

Resource links provided by NLM:

Further study details as provided by PTC Therapeutics:

Primary Outcome Measures:
  • CFTR activity as assessed by nasal transepithelial potential difference (TEPD)

Secondary Outcome Measures:
  • Side effects
  • Presence of CFTR protein and mRNA
  • Compliance with treatment
  • Lung function
  • Sputum test
  • PTC124 pharmacokinetics
  • Changes in CF symptoms
  • Changes in weight
  • Changes in cough

Estimated Enrollment: 23
Study Start Date: December 2006
Study Completion Date: July 2007
Detailed Description:

In this study, the same patients who were enrolled in a previous study of PTC124 (protocol number PTC124-GD-005-CF) will be treated with the investigational drug PTC124 for a period of 84 days. Evaluation procedures (history, physical examination, blood and urine tests to assess organ function, electrocardiogram (ECG), chest x-ray, and CF-specific tests) to determine if a patient qualifies for the study will be performed within 21 days prior to the start of treatment. Eligible patients who elect to enroll in the study will be assigned to receive PTC124 treatment (based upon TEPD results from the previous study) at 1 of the 2 following dose levels:

  • PTC124, given 3 times per day (TID) with a regimen of 4 mg/kg at breakfast, 4 mg/kg at lunch, and 8 mg/kg at dinner, or
  • PTC124, given 3 times per day (TID) with a regimen of 10 mg/kg at breakfast, 10 mg/kg at lunch and 20 mg/kg at dinner.

During the study, PTC124 efficacy, safety, and pharmacokinetics will be evaluated periodically with measurement of transepithelial potential difference (TEPD), medical history, physical examinations, blood tests, urinalysis, sputum tests, ECGs, chest x-ray, and pulmonary function tests.


Ages Eligible for Study:   18 Years and older   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No

Inclusion Criteria:

Patients must meet all of the following conditions to be eligible for enrollment into the study:

  • Completion of ≥ 1 cycle of PTC124 treatment in the previous Phase 2a study protocol (Protocol Number PTC124-GD-005-CF).
  • Abnormal nasal epithelial TEPD total chloride conductance (a more electrically negative value than -5 mV for Δchloride-free+isoproterenol).
  • FEV1 ≥ 40% of predicted for age, gender, and height.
  • Oxygen saturation (as measured by pulse oximetry) ≥92% on room air.
  • Willingness of male and female patients, if not surgically sterile, to abstain from sexual intercourse or employ a barrier or medical method of contraception during the study drug administration and follow-up periods.
  • Negative pregnancy test (for females of childbearing potential).
  • Willingness and ability to comply with scheduled visits, drug administration plan, study procedures, and study restrictions.
  • Ability to provide written informed consent.
  • Evidence of signed and dated informed consent document indicating that the patient has been informed of all pertinent aspects of the trial.

Exclusion Criteria:

The presence of any of the following conditions will exclude a patient from enrollment in the study:

  • Prior or ongoing medical condition, medical history, physical findings, ECG findings, or laboratory abnormality that, in the investigator's opinion, could adversely affect the safety of the patient, makes it unlikely that the course of treatment or follow-up would be completed, or could impair the assessment of study results.
  • Ongoing acute illness including acute upper or lower respiratory infections within 2 weeks before start of study treatment in this study.
  • History of major complications of lung disease (including recent massive hemoptysis or pneumothorax) within 2 months prior to start of study treatment in this study.
  • Abnormalities on screening chest x-ray suggesting clinically significant active pulmonary disease other than CF, or new, significant abnormalities such as atelectasis or pleural effusion which may be indicative of clinically significant active pulmonary involvement secondary to CF.
  • Hemoglobin <10 g/dL.
  • Serum albumin <2.5 g/dL.
  • Abnormal liver function (serum total bilirubin > the upper limit of normal, or serum ALT, AST, or GGT >2.0 times the upper limit of normal). Abnormal renal function (serum creatinine >1.5 times upper limit of normal).
  • Pregnancy or breast-feeding.
  • History of solid organ or hematological transplantation.
  • Exposure to another investigational drug within 14 days prior to start of study treatment in this study.
  • Ongoing participation in any other therapeutic clinical trial.
  • Ongoing use of thiazolidinedione peroxisome proliferator-activated receptor gamma (PPAR γ) agonists, eg, rosiglitazone (Avandia® or equivalent) or pioglitazone (Actos® or equivalent).
  • Change in intranasal medications (including use of corticosteroids, cromolyn, ipratropium bromide, phenylephrine, or oxymetazoline) within 7 days prior to start of study treatment in this study.
  • Change in treatment with systemic or inhaled corticosteroids within 7 days prior to start of study treatment in this study.
  • Use of or requirement for inhaled gentamicin or amikacin within 14 days prior to start of study treatment in this study or during study treatment.
  • Requirement for systemic aminoglycoside antibiotics within 14 days prior to start of study treatment in this study.
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Please refer to this study by its identifier: NCT00351078

Hadassah University Hospital - Mount Scopus
Jerusalem, Israel, 91240
Sponsors and Collaborators
PTC Therapeutics
Principal Investigator: Eitan Kerem, MD Hadassah University Hospital - Mount Scopus
  More Information

Additional Information:
Publications automatically indexed to this study by Identifier (NCT Number): Identifier: NCT00351078     History of Changes
Other Study ID Numbers: PTC124-GD-005e-CF
Study First Received: July 11, 2006
Last Updated: October 16, 2007

Keywords provided by PTC Therapeutics:
Cystic fibrosis
Nonsense mutation
Premature stop codon

Additional relevant MeSH terms:
Cystic Fibrosis
Pathologic Processes
Pancreatic Diseases
Digestive System Diseases
Lung Diseases
Respiratory Tract Diseases
Genetic Diseases, Inborn
Infant, Newborn, Diseases processed this record on April 26, 2017