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Use of Biopsy Punch for Skin Biopsies for Fibroblasts Culturing

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ClinicalTrials.gov Identifier: NCT00340912
Recruitment Status : Completed
First Posted : June 21, 2006
Last Update Posted : July 2, 2017
Sponsor:
Information provided by:
National Institutes of Health Clinical Center (CC)

Brief Summary:
Fibroblasts can be cultured from human skin biopsies using fetal bovine serum and artificial growth medium. Fibroblasts cultured in this way retain the genetic characteristics of their donor but can be used for experiments where study conditions are much more precisely controlled than they can be in in vivo studies. We plan to culture fibroblasts from Pima Indians for two purposes. Firstly, to look for an in vitro system to measure insulin resistance, the underlying abnormality in Pima diabetics. Secondly, to look for biochemical markers of diabetes or obesity in Pimas employing two dimensional electrophoresis techniques.

Condition or disease
Fibroblasts

Detailed Description:
Fibroblasts can be cultured from human skin biopsies using fetal bovine serum and artificial growth medium. Fibroblasts cultured in this way retain the genetic characteristics of their donor but can be used for experiments where study conditions are much more precisely controlled than they can be in in vivo studies. We plan to culture fibroblasts from Pima Indians for two purposes: 1) to look for an in vitro system to measure insulin resistance, the underlying abnormality in Pima diabetics; and 2) to look for biochemical markers of diabetes or obesity in Pimas employing two-dimensional electrophoresis techniques.

Study Type : Observational
Estimated Enrollment : 500 participants
Official Title: Use of Biopsy Punch for Skin Biopsies for Fibroblasts Culturing
Study Start Date : December 7, 1988
Primary Completion Date : December 7, 1988

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Biopsy
U.S. FDA Resources





Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 50 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Criteria
  • INCLUSION CRITERIA:

We will include Pima Indians who do not meet any of the exclusion criteria.

EXCLUSION CRITERIA:

We will exclude any seriously ill individuals, any known to have a bleeding diathesis or to be immunosuppressed or subjects who are carriers of HIV or HBV.

Children will be excluded, as the age range for this study's participants is 18-50.

Pregnant women will be excluded on the basis of a urinary pregnancy test.

All other subjects are potentially included.


Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00340912


Locations
United States, Arizona
NIDDK, Phoenix
Phoenix, Arizona, United States, 85014
Sponsors and Collaborators
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

ClinicalTrials.gov Identifier: NCT00340912     History of Changes
Other Study ID Numbers: 9999880215
OH88-DK-0215
First Posted: June 21, 2006    Key Record Dates
Last Update Posted: July 2, 2017
Last Verified: October 1, 2009

Keywords provided by National Institutes of Health Clinical Center (CC):
Skin Biopsy