Regulation of the Release of Inflammatory Mediators From Blood Leukocytes
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|ClinicalTrials.gov Identifier: NCT00180765|
Recruitment Status : Completed
First Posted : September 16, 2005
Last Update Posted : August 25, 2015
|Condition or disease||Intervention/treatment|
|Chronic Obstructive Airway Disease Healthy Volunteers||Procedure: Up to 100ml blood will be taken by venupuncture.|
The aim of this study is to investigate the mechanisms that regulate the survival of blood leukocytes as well as the synthesis and release of inflammatory mediators from cells from normal individuals and subjects with COPD. The hypothesis is that in COPD the life-span of leukocytes, such as the neutrophil, is enhanced and this may contribute to inflammation, a prominent characteristic of this disease, by secreting and releasing inflammatory mediators. We also suggest that differences may exist in the sensitivity of the various leukocytes to different therapies.
Leukocytes will be purified from the peripheral venous blood of patients with COPD as well as healthy individuals. We will then investigate the effects of novel and existing therapeutic agents on leukocyte survival and inflammatory mediator synthesis and release. We will also examine the regulation and release of enzymes known to damage lung tissue. Further studies will be carried out to elucidate the signal transduction pathways that lead to the activation, altered longevity and function of leukocytes. In other experiments ribonucleic acids or RNA may be extracted form leukocytes to investigate which genes are involved. The primary objective is to identify the mechanisms that enhance leukocyte longevity and inflammatory mediator and/or enzyme synthesis and release with a view to identifying novel targets for drug therapy.
|Study Type :||Observational|
|Enrollment :||30 participants|
|Official Title:||Regulation of the Release of Inflammatory Mediators From Blood Leukocytes: A Comparison of Healthy Subjects, Healthy Smokers and Patients With COPD.|
|Study Start Date :||October 2001|
|Actual Primary Completion Date :||February 2007|
|Actual Study Completion Date :||February 2008|
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00180765
|National Heart & Lung Institute, Imperial College|
|Chelsea, London, United Kingdom, SW3 6LY|
|Principal Investigator:||Peter J Barnes, DSc||National Heart & Lung Institute, Imperial College|