Cytokine Regulation of Natural Killer Receptors in Inhibiting Activated T Cell Function

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00173290
Recruitment Status : Unknown
Verified June 2005 by National Taiwan University Hospital.
Recruitment status was:  Recruiting
First Posted : September 15, 2005
Last Update Posted : June 3, 2008
Information provided by:
National Taiwan University Hospital

Brief Summary:
In this study proposal, the investigators will extend their previous studies and examine the kinetic cytotoxic activity with concordant expression of inhibitory natural killer (NK) receptors (iNKR) on activated T cells. The inhibitory role of cytokines will be defined by utilizing the investigators' previously established models of mixed lymphocytes and tumor cells coculture to analyze the expression and activity of cytokines involved in the regulation of iNKRs on cancer-encountered T cells.

Condition or disease
Cervical Cancer Breast Cancer Endometrial Cancer Cancer

Detailed Description:
In our extended studies, we have directly examined the expressions of various inhibitory natural killer cell Receptors (iNKRs) on Tumor-infiltrating lymphocytes (TILs) derived from human Cervical cancer (CC) by triple-color flow cytometry with combination of different surface markers. We found up-regulated expression of certain iNKRs (CD94/NKG2A) in TILs and in mixed lymphocyte-cancer cell cocultures. In our previous studies, we demonstrated that certain cytokines, including IL-10, TGF-beta (Sheu et al, Journal of Immunology, 2001, 167:2972-2978), and IL-15 (Sheu et al, Cancer Research, 2005, 65:2921-2929) can be expressed by CC cells. We further demonstrated that activated T cells bear iNKRs which inhibit cytotoxic activity. iNKRs are proposed to restrain the T cell receptor (TCR)-mediated cytolysis. We found that CC cells had altered HLA-A, -B, and -C molecules in a cancer microenvironment. The acquisition of both NK-like activity and expression of iNKRs by these T cells is parallel to prevent damage to normal host cells. However, there is also limited knowledge about the regulatory role of iNKR expression in T cell cytotoxicity.

Study Type : Observational
Estimated Enrollment : 200 participants
Study Start Date : July 2004
Estimated Study Completion Date : December 2006

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Ages Eligible for Study:   18 Years to 50 Years   (Adult)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Cancer patients
  • Healthy volunteers

Exclusion Criteria:

  • Immunosuppression
  • HIV carrier

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To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00173290

Contact: Bor-Ching Sheu, MD, PhD 886-2-2312-3456 ext 5559

National Taiwan University Hospital Recruiting
Taipei, Taiwan, 100
Contact: Bor-Ching Sheu, MD, PhD    886-2-2312-3456 ext 5559   
Sponsors and Collaborators
National Taiwan University Hospital
Principal Investigator: Bor-Ching Sheu, MD, PhD Department of Obstetrics and Gynecology, National Taiwan University Hospital

Publications of Results: Identifier: NCT00173290     History of Changes
Other Study ID Numbers: 9461700613
NTUH 94-S045
NTUH 95-0399
First Posted: September 15, 2005    Key Record Dates
Last Update Posted: June 3, 2008
Last Verified: June 2005

Keywords provided by National Taiwan University Hospital:
Cervical cancer
Breast cancer
T lymphocyte
NK receptor

Additional relevant MeSH terms:
Uterine Cervical Neoplasms
Endometrial Neoplasms
Uterine Neoplasms
Genital Neoplasms, Female
Urogenital Neoplasms
Neoplasms by Site
Uterine Cervical Diseases
Uterine Diseases
Genital Diseases, Female