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Safety, Tolerability and Immunogenicity of Recombinant Anthrax Vaccine Compared With Anthrax Vaccine Adsorbed

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00170469
Recruitment Status : Completed
First Posted : September 15, 2005
Last Update Posted : September 15, 2008
National Institute of Allergy and Infectious Diseases (NIAID)
Information provided by:
PharmAthene UK Limited

Brief Summary:
This is a dose ranging study comparing different vaccine schedules of rPA vaccine, for Anthrax, to the licensed dose of AVA, another Anthrax vaccine. Safety and the capability to induce an immune response will be evaluated.

Condition or disease Intervention/treatment Phase
Bacillus Anthracis (Anthrax) Biological: AVA Biological: rPA vaccine containing alhydrogel Phase 2

Detailed Description:
Anthrax is a zoonotic disease, occurring in wild and domestic mammals, caused by the spore-forming bacterium Bacillus anthracis (B. anthracis). Anthrax occurs in humans when they are exposed to infected animals, tissue from infected animals or when they are directly exposed to B. anthracis or its spores. Depending on the route of infection, anthrax disease can occur in three forms: cutaneous, gastrointestinal, and inhalation. In the United States of America (USA), the annual incidence of human anthrax has declined from approximately 130 cases annually in the early 1900s to no cases during 1999 to 2000. However, in the USA, shortly after September 11th, 2001, there were 22 cases (18 confirmed) of inhaled and cutaneous anthrax infection that were related to contaminated mail. The development of a new anthrax vaccine is necessary because the current AVA vaccine requires the growth of B. anthracis in its manufacturing process and has a complex administration regimen of six administrators of vaccine in 18 months. As protective antigen (PA) is a central virulence factor in anthrax pathogenesis and a major immunogen in the current vaccine, a recombinant, acellular, protective antigen-based anthrax vaccine, could offer an improved manufacturing process and a simpler dosing schedule. Furthermore, the vaccine could offer improved protection against inhaled B. anthracis, and could, when used in conjunction with antibiotics, form part of the management of anthrax exposed individuals. This is a dose ranging study comparing different primary vaccine schedules of rPA Anthrax vaccine to the licensed dose of Anthrax Vaccine Adsorbed. The study is designed to measure the immune response, and to evaluate the safety and tolerability of different doses of rPA Anthrax Vaccine. After subjects have given informed consent, they will undergo physical exams, medical history screening, pregnancy tests, ECG, HIV, Hepatitis B & C tests, safety blood tests, and urine drug and alcohol screen. Subjects will be asked to complete a diary card to record any symptoms they may experience. The subjects that receive AVA will be offered the opportunity to complete the licensed course of vaccination which would involve AVA vaccinations at 6, 12 and 18 months.

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 226 participants
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single (Participant)
Primary Purpose: Prevention
Official Title: A Phase II, Dose Ranging Multi-Centre, Single Blind, Parallel-Group, Controlled Study of the Safety, Tolerability and Immunogenicity of Recombinant (rPA Based) Anthrax Vaccine Compared With Anthrax Vaccine Adsorbed in a Healthy Population
Study Start Date : March 2005
Actual Primary Completion Date : February 2007
Actual Study Completion Date : February 2007

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Anthrax

Arm Intervention/treatment
Experimental: 1
Low dose rPA vaccine
Biological: rPA vaccine containing alhydrogel
Experimental: 2
High dose rPA vaccine
Biological: rPA vaccine containing alhydrogel
Active Comparator: 3
Active vaccine control
Biological: AVA

Primary Outcome Measures :
  1. Safety of rPA vaccine

Secondary Outcome Measures :
  1. Immunogenicity of rPA vaccine

Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 55 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  1. Healthy males or females.
  2. Aged between 18-55 years (inclusive).
  3. A body mass index (BMI) of 18-35.
  4. Signed informed consent, which includes information about the potential risks and effects of rPA and AVA.
  5. A medical history without major organ pathology (e.g. cardiac, immunological, psychiatric, endocrine or neurological disorders, cancer or other wasting diseases - (adequately treated actinic keratosis, or basal cell carcinoma [BCC], or carcinoma in situ [CIS] of the cervix are permitted).
  6. A female may be enrolled if one of the following criteria applies:

    Either If of child-bearing capacity then: A female is not pregnant or breast feeding AND is routinely using adequate injectable or transdermal (administered at the recommended frequency) or oral contraception (at a stable dose for at least three months prior to the first dose of vaccine) and will continue to do so during the study, augmenting this contraceptive measure with a barrier method OR is sexually abstinent OR is monogamous and has a partner who has had a vasectomy (>1 month previously) OR is using a commonly recognized copper and hormone implanted intrauterine device (IUD) such as TCu-380A, TCu-220C, MLCu-375, Nova-T, or LNG-20. In addition, the subject must have a negative blood pregnancy test prior to enrolment into the study and negative urine pregnancy test pre-dose.

    Or A female is post menopausal (defined as a female with no menstrual cycle for at least 24 months and of menopausal age (>45 years) Or A female with no menstrual cycle for between 12 and 24 months and of menopausal age (>45 years) who has a negative blood pregnancy test prior to enrolment into the study and a negative urine pregnancy test pre-dose.

    Or A female has been surgically sterilized (confirmed by review of medical record).

    Or A female has had a total hysterectomy at least 3 months prior to the start of the study (confirmed by review of medical record).

  7. A male may be enrolled if willing to use barrier methods of contraception and whose partner is using an acceptable form of contraception for 3 months post each dose.

Exclusion Criteria:

  1. Presence of any clinically significant medical condition as determined by the Investigator.
  2. Medically significant hypersensitivity or idiosyncratic reaction related to any medical product including vaccines.
  3. History or evidence of drug abuse 1 year prior to enrollment.
  4. Participation in a clinical study of an investigational vaccine within 3 months prior to the start of the study or an investigational drug product within 30 days prior to the start of the study.
  5. Use of any prescription or non-prescription medication within 7 days prior to the first dosing with the exception of over-the-counter (OTC) antihistamine, non-steroidal anti-inflammatory drugs (NSAID), acetaminophen, OTC decongestants or oral/injectable/transdermal contraceptives. Any medication taken within 7 days of the first dosing will be recorded.
  6. History or suspicion of inability to co-operate adequately.
  7. Donation of blood or blood products for a period of 4 weeks prior to participation in the study.
  8. Immunodeficiency or clinically active autoimmune disease.
  9. Positive urine alcohol and drug screen for drugs of abuse (opiates, methadone, cocaine, amphetamines, cannabinoids, and barbiturates).
  10. Positive test for human immunodeficiency virus (HIV), and/or hepatitis B and/or hepatitis C.
  11. Vaccination(s) with a live vaccine in the previous 4 weeks or killed / inactivated vaccines in the previous 3 weeks.
  12. Blood or plasma transfusions, or pooled gamma-globulin in the previous 3 months and need for blood or plasma transfusions during this study.
  13. Received anthrax vaccine or anthrax immune globulin or been otherwise exposed to B. anthracis.
  14. Clinically relevant abnormal findings on routine physical examination.
  15. Clinically significant out-of-range laboratory tests at screening including: urinalysis, serum creatine, lactate dehydrogenase (LDH), potassium, glucose, liver function tests (LFT); absolute neutrophil count, platelet count, white blood cell count, electrolytes, clotting and blood hemoglobin.
  16. Twelve-lead ECG recording with clinically relevant signs of pathology and conduction disturbances as judged by the investigator.
  17. Presence of tattoos that cover or partially cover the injection sites on the upper arm.
  18. Known sensitivity to Latex.

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00170469

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United States, Alabama
Greater Huntsville Family Practice, PC
Huntsville, Alabama, United States, 35802
Discovery Alliance, Inc.
Mobile, Alabama, United States, 36606
United States, California
San Diego, California, United States, 92108
United States, District of Columbia
Washington, District of Columbia, United States, 20006
United States, Florida
Florida Medical Research Institute
Gainesville, Florida, United States, 32607
Melbourne, Florida, United States, 32935
Miami Research Associates
South Miami, Florida, United States, 33143
United States, Illinois
Orland Park, Illinois, United States, 60462
United States, Indiana
South Bend, Indiana, United States, 46601
United States, Oklahoma
Oklahoma City, Oklahoma, United States, 73112
Lynn Health Science Institute
Oklahoma City, Oklahoma, United States, 73120
United States, Tennessee
McKenzie Medical Center
McKenzie, Tennessee, United States, 38201
United States, Texas
PharamTex Research, Inc.
Amarillo, Texas, United States, 79106
Houston, Texas, United States, 77024
United States, Virginia
Metropolitan Research
Fairfax, Virginia, United States, 22031
Carilion Medical Associates
Galax, Virginia, United States, 24333
Sponsors and Collaborators
PharmAthene UK Limited
National Institute of Allergy and Infectious Diseases (NIAID)
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Principal Investigator: Lawrence Currie, MD Accelovance

Layout table for additonal information Identifier: NCT00170469     History of Changes
Other Study ID Numbers: 05-0002
First Posted: September 15, 2005    Key Record Dates
Last Update Posted: September 15, 2008
Last Verified: September 2008
Keywords provided by PharmAthene UK Limited:
Additional relevant MeSH terms:
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Bacillaceae Infections
Gram-Positive Bacterial Infections
Bacterial Infections
Aluminum Hydroxide
Immunologic Factors
Physiological Effects of Drugs
Adjuvants, Immunologic
Molecular Mechanisms of Pharmacological Action
Gastrointestinal Agents