Biomarkers of iNOS Activity in COPD, Asthma, Healthy Control
This pilot study will measure the baseline levels and the intra- and inter-subject variability of exhaled (alveolar and bronchial) and nasal NO in smoking subjects with mild and moderate COPD on or off steroid treatment, smoking healthy volunteers and non-smoking mild asthmatics off steroids, using multiple exhalation flow rates.
In addition, exploratory markers of iNOS activity (eg. 3-nitrotyrosine, nitrite, nitrate and 3-nitro-4-hydoxyphenylacetic acid) in blood, sputum, urine and exhaled breath condensate will be evaluated. We will also evaluate exhaled and nasal NO in a population of mild asthmatics in order to obtain baseline and variability data for future clinical studies involving asthmatic subjects.
|Healthy Smokers Healthy Non-Smokers Patients With Mild Asthma Patints With COPD (GOLD II)||Procedure: Induced Sputum Procedure: Exhaled Nitric Oxide Procedure: Nasal Nitric Oxide Procedure: Blood Tests Procedure: 24hr Urine Samples Procedure: Exhaled Breath Condensate Procedure: Spirometry|
|Study Design:||Observational Model: Defined Population
Time Perspective: Cross-Sectional
Time Perspective: Prospective
|Official Title:||A Pilot Study to Evaluate and Develop Biomarkers of iNOS Activity in Patients With COPD or Asthma and Healthy Volunteers|
Please refer to this study by its ClinicalTrials.gov identifier: NCT00159302
|Section of Airway Disease, Asthma Lab, Imperial College London, Royal Brompton Hospital|
|London, United Kingdom, SW3 6LY|
|Principal Investigator:||Sergei A Kharitonov, MD PhD||Imperial College London|