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Amino Acids in Urine, Diet, and Blood Pressure: International Population Study

This study has been completed.
Information provided by:
National Heart, Lung, and Blood Institute (NHLBI) Identifier:
First received: October 12, 2000
Last updated: March 15, 2016
Last verified: December 2005
To analyze the relationship of dietary variables to urinary excretion of amino acids and the relationships of specific urinary amino acids to blood pressure.

Cardiovascular Diseases Heart Diseases Hypertension

Study Type: Observational

Further study details as provided by National Heart, Lung, and Blood Institute (NHLBI):

Study Start Date: August 2000
Study Completion Date: July 2005
Detailed Description:


The study analyzes data from the INTERMAP study, an international, cooperative, 17-sample population study of 4,706 men and women ages 40-59 in the United States, the United Kingdom, China, and Japan. It will provide the largest amount of data available on the patterns of urinary amino acid excretion. It will also make it possible to determine if the excretion of specific amino acids are related to the intake of dietary protein from specific food sources. Furthermore, it will provide the ability to test the hypotheses that intake of dietary protein is related to levels of both systolic and diastolic blood pressure. These hypotheses are strongly held in Japan, one of the countries participating in the INTERMAP study, and have only been investigated to a limited degree in the United States and Europe.


The purpose of the study was to analyze data from the INTERMAP Study, an international, cooperative, 17-sample population study of 4,706 men and women ages 40-59 in four countries including the United States, the United Kingdom, China, and Japan. Analyses assessed the following: whether, independent of multiple possible confounding variables, there were significant relationships of dietary protein (total, animal, vegetable) and of dietary specific amino acids (AA) to 24-hour urinary excretion of total and individual AA and related compounds; systolic and diastolic blood pressure (BP) of individuals were related to their 24-hour urinary excretion of taurine -- a possible marker of fish protein intake; 1-methylhistidine, 3-methylhistidine, histidine, lysine, anserine (1-methylhistidine-B-alanine dipeptide) -- reflecting meat protein intake; arginine -- source in cells for nitric oxide (NO), implicated in vasodilation; other urinary amino acids.

INTERMAP has already collected standardized comprehensive dietary and blood pressure data on all individuals in its 17 samples (four 24-hour dietary recalls/person yielding data on 60+ nutrients, including 19 amino acids, two 24-hour urine specimens/person with data on Na, K, urea N, creatinine, volume; eight standardized systolic blood pressure/diastolic blood pressure measurements/person; other data). Urinary concentrations of amino acids were analyzed chromatographically by high performance automatic amino acid analyzers in the INTEPMAP Central Laboratory in Leuven, Belgium. Regression coefficients for amino acid-blood pressure relationships were computed without and with correction for reliability (regression-dilution bias); sample-by-sample with pooling by country, region, and then overall; also by gender; for persons not on antihypertensive treatment; nonhypertensives; those free of a history of recent diet change; by education; to evaluate comprehensively whether urinary amino acid excretion could add meaningfully to dietary data to give a better total understanding of relationships of dietary protein-amino acids to blood pressure.

The study completion date listed in this record was obtained from the "End Date" entered in the Protocol Registration and Results System (PRS) record.


Ages Eligible for Study:   up to 100 Years   (Child, Adult, Senior)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   No
No eligibility criteria
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Please refer to this study by its identifier: NCT00006406

Sponsors and Collaborators
National Heart, Lung, and Blood Institute (NHLBI)
OverallOfficial: Martha Daviglus Northwestern University